Core Facility Flow Cytometry
Core Facility Flow Cytometry (CF-FLOW) provides state of the art infrastructure, expert support and service for a wide range of cell sorting and analytical flow cytometry applications. Our high- end cytometers allow measuring up to 30 parameters while our spatial biology solution can unravel hundreds of protein-and RNA markers on tissue sections.
To keep the facility at the cutting edge of science we continuously develop and implement new methods, also for cross-platform workflows with other Helmholtz Munich cores, and we are open to test new technological developments. We offer comprehensive support including experimental design, troubleshooting and data analysis and are open to internal and external customers. A complimentary portfolio of basic and advanced training courses and support with grants and publications help our users in achieving their goals.
We use the iLab Core Management system to book services by internal and external users.
Core Facility Flow Cytometry (CF-FLOW) provides state of the art infrastructure, expert support and service for a wide range of cell sorting and analytical flow cytometry applications. Our high- end cytometers allow measuring up to 30 parameters while our spatial biology solution can unravel hundreds of protein-and RNA markers on tissue sections.
To keep the facility at the cutting edge of science we continuously develop and implement new methods, also for cross-platform workflows with other Helmholtz Munich cores, and we are open to test new technological developments. We offer comprehensive support including experimental design, troubleshooting and data analysis and are open to internal and external customers. A complimentary portfolio of basic and advanced training courses and support with grants and publications help our users in achieving their goals.
We use the iLab Core Management system to book services by internal and external users.
Useful Links and Resources
Below are just a few of many more links for a general introduction to flow cytometry:
An introduction to flow cytometry by M. Ormerod (https://flowbook.denovosoftware.com/)
BD Biosciences Introduction to Flow cytometry (self-paced modules)
www.bdbiosciences.com/en-us/learn/training/basic/flow-cytometry-introduction
BD Biosciences Multicolor Flow Cytometry tools
https://www.bdbiosciences.com/en-ch/learn/applications/multicolor-flow-cytometry#Overview
BD Biosciences Seminar series “Understanding the immune system” (registration required, especially good for high parameter multicolor flow cytometry)
lp.bd.com/202012-BDB21-UKI_EN-Immune_Systems-LP_LP-EN-01-MainLP.html
Biolegend WebTools for Flow Cytometry
https://www.biolegend.com/en-us/web-tools-tab
Biorad Flow Cytometry explained
https://www.bio-rad-antibodies.com/flow-cytometry-explained.html
ThermoFisher Flow cytometry Learning Center
https://www.thermofisher.com/de/de/home/life-science/cell-analysis/flow-cytometry/flow-cytometry-learning-center.html
FlowJo tutorials and webinars
https://www.flowjo.com/learn/flowjo-university/flowjo
Expert Bio/The cheeky Scientist (Blog)
https://expert.cheekyscientist.com/blog/
BiteSize Bio/Flow Cytometry (Blog)
https://bitesizebio.com/technical-channels/flow-cytometry/
Here just a selection of many more useful tools:
BD Spectrum Viewer
https://www.bdbiosciences.com/en-us/resources/bd-spectrum-viewer
BD Panel design resource collection
https://www.bdbiosciences.com/en-us/resources/panel-design#Overview
Biolegend Spectra Analyzer
https://www.biolegend.com/spectraanalyzer
Biolegend Multicolour staining guide
https://www.chromocyte.com/Educate/Resource/biolegend-multicolor-staining-guide
Biorad Spectra Viewer
https://www.bio-rad-antibodies.com/fluorescent-spectraviewer.html