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Multiparameter Analytical Flow Cytometry

Flow cytometry allows the fast, high-throughput multiparameter analysis the of physical and (bio-) chemical properties of cells and/or particles within a heterogeneous sample. Single cell in suspension are typically labelled with fluorescent markers (antibodies and dyes) against cell surface and/or intracellular targets and passed through a focused laser beam. Resulting scattered and fluorescent light is collected and transformed into electrical signals for quantification and data storage. The number of possible parameters measured depends on the number of lasers and detectors as well as that of available and detectable fluorochromes.

CF-FLOW at Helmholtz Munich (new core in Q3/2024) currently offers high dimensional multiparameter analysis of up to 30 parameters on a BD FACS Symphony A3 with autoloader (Click  here for instrument configuration and HTS quick start guide).

Access to self-service instruments of CF-FLOW is possible for trained and approved users only while operator run services can be booked by all registered researchers. New users to CF-FLOW have to sign up via ilab and book relevant training (course times are listed on ilab). Basic flow cytometry training is mandatory for all independent use of our facility.  Prior to starting a new project users can book a free consultation with CF-FLOW to obtain guidance on best practice for their experiments.

CF-FLOW follows the general Terms of use for Helmholtz Munich Core facilities, additional rules for the use of the flow core will be discussed prior to first use of our services. Please note that formalities are required in advance for working with certain samples such as primary human material or genetically modified organisms (GMO).

Flow cytometry allows the fast, high-throughput multiparameter analysis the of physical and (bio-) chemical properties of cells and/or particles within a heterogeneous sample. Single cell in suspension are typically labelled with fluorescent markers (antibodies and dyes) against cell surface and/or intracellular targets and passed through a focused laser beam. Resulting scattered and fluorescent light is collected and transformed into electrical signals for quantification and data storage. The number of possible parameters measured depends on the number of lasers and detectors as well as that of available and detectable fluorochromes.

CF-FLOW at Helmholtz Munich (new core in Q3/2024) currently offers high dimensional multiparameter analysis of up to 30 parameters on a BD FACS Symphony A3 with autoloader (Click  here for instrument configuration and HTS quick start guide).

Access to self-service instruments of CF-FLOW is possible for trained and approved users only while operator run services can be booked by all registered researchers. New users to CF-FLOW have to sign up via ilab and book relevant training (course times are listed on ilab). Basic flow cytometry training is mandatory for all independent use of our facility.  Prior to starting a new project users can book a free consultation with CF-FLOW to obtain guidance on best practice for their experiments.

CF-FLOW follows the general Terms of use for Helmholtz Munich Core facilities, additional rules for the use of the flow core will be discussed prior to first use of our services. Please note that formalities are required in advance for working with certain samples such as primary human material or genetically modified organisms (GMO).