IBMI Fluorescence Imaging

Gorpas lab

Our research focuses on ground-breaking solutions for unmet needs in medicine and biology. Specifically, we investigate the potential of fluorescence and optical imaging as well as spectroscopy for providing

1) insights into the specific molecular changes underlying the process of field cancerization,

2) means of earlier, faster, and personalized disease detection, and

3) imaging-assisted surgical planning and intraoperative guidance.

Go to Dr. Dimitris Gorpas

Gorpas lab

1) insights into the specific molecular changes underlying the process of field cancerization,

2) means of earlier, faster, and personalized disease detection, and

3) imaging-assisted surgical planning and intraoperative guidance.

Go to Dr. Dimitris Gorpas

Our research focus lies within the investigation and development of ground breaking solutions for medical and biological questions that go well beyond conventional "photographic" fluorescence and optical imaging and spectroscopy. Some examples of our research are:

We work closely with clinicians to develop real-time fluorescence molecular imaging and endoscopic technologies for earlier, faster, and personalized disease detection and management. This research is geared toward novel multi-spectral instrumentation and the use of appropriate fluorescent agents with clinical relevance. We intensively explore inflammation and gastrointestinal cancers, as early detection is anticipated to have a tremendous impact on disease prognosis and healthcare. Towards this end, we have been awarded grants to systematically explore the added value of fluorescence molecular endoscopy and optical spectroscopy to standard clinical practice.

Our group is one of the leading centers worldwide that has systematically been working towards the high-fidelity fluorescence molecular imaging. We are closely collaborating with numerous groups to enable comparative clinical studies, optimized development of imaging systems and data visualization platforms, and performance assessment and quality control of existing ones. Our vision is the establishment of standardization protocols and phantoms that will ensure the translation of fluorescence molecular imaging and endoscopy into the clinics.

Intravascular ultrasound and near-infrared fluorescence (IVUS-NIRF) is a technology with tremendous potential for becoming a valuable asset for interventional cardiology. Our group works closely with cardiologists and biologists to identify new biomarkers that could lead to faster and personalized cardiovascular diagnostics. We are actively working for the development of more sensitive detection systems, miniaturized catheters, and algorithms that would objectively highlight disease-related morphological and biological alterations in real-time.

We develop robust, multi-spectral approaches for automatic imaging of tissues during serial cryosectioning. Our current research pursues hardware optimization and development of imaging methods to correct for photon scattering and provide quantitative readouts that are consistent with the true biodistribution of fluorescent tracers. Additionally, in close collaboration with other groups, we employ multi-spectral cryo-fluorescence tomography for providing insights in the distribution and kinetics of novel pharmaceuticals and clinically relevant biomarkers.

We have also developed a robust hybrid fluorescence molecular tomography (FMT) and X-ray computed tomography (XCT) imaging system for quantitative in vivo molecular tomography in small animals. Through this work we enable in vivo longitudinal studies for better understanging the temporal biodistribution of pharmaceuticals and new tracers. In addition, we systematically investigate biologically relevant questions and drug discovery, while also improving current technology.

In line with our main research goal to provide solutions for earlier, faster, and personalize disease diagnosis and management, we are developing label-free microscopy technologies and statistical tools to measure and analyze data from numerous modalities, including Raman, two photon excitation fluorescence, second harmonic generation, etc. We are very interested in identifying the optimal combination of modalities that would increase our confidence for the detection of numerous clinically relevant conditions and to better understand the contribution of different clinically relevant biomarkers.

Keizers, B. ; Stroet, M.C.M. ; Ali, M. ; Floru, S. ; Saliën, J. ; Mezzanotte, L. ; Delikatny, E.J. ; Gibbs, S.L. ; Giuliani, S. ; Gioux, S. ; Ingelberts, H. ; Kruijff, S. ; Ntziachristos, V. ; LaRochelle, E. ; Rogalla, S. ; Rosenthal, E.L. ; Samkoe, K.S. ; Tichauer, K.M. ; Vahrmeijer, A.L. ; Witjes, M.J.H. ; Voskuil, F.J. ; Gorpas, D. ; Hernot, S. ; van der Zaag, P.J.

Reflect: Reporting guidelines for preclinical, translational and clinical fluorescence molecular imaging studies.

Kriukova, E. ; Mazurenka, M. ; Marcazzan, S. ; Tschurtschenthaler, M. ; Puppels, G.J. ; Glasl, S. ; Saur, D. ; Jesinghaus, M. ; Pouliou, M. ; Agelopoulos, M. ; Klinakis, A. ; Quante, M. ; Ripoll, J. ; Ntziachristos, V. ; Gorpas, D.

Probing field cancerization in the gastrointestinal tract using a hybrid raman and partial wave spectroscopy microscope.

Gorpas, D. ; Ntziachristos, V.

Multi-parametric standards for performance assessment and quality control of fluorescence molecular imaging and endoscopy systems.

Keizers, B. ; Nijboer, T.S. ; van der Fels, C.A.M. ; van den Heuvel, M.C. ; van Dam, G.M. ; Kruijff, S. ; Jan de Jong, I. ; Witjes, M.J.H. ; Voskuil, F.J. ; Gorpas, D. ; Browne, W.R. ; van der Zaag, P.J.

Systematic comparison of fluorescence imaging in the near-infrared and shortwave-infrared spectral range using clinical tumor samples containing cetuximab-IRDye800CW.

Kriukova, E. ; LaRochelle, E. ; Pfefer, T.J. ; Kanniyappan, U. ; Gioux, S. ; Pogue, B.W. ; Ntziachristos, V. ; Gorpas, D.

Impact of signal-to-noise ratio and contrast definition on the sensitivity assessment and benchmarking of fluorescence molecular imaging systems.

Huang, Y.J. ; Rieder, J. ; Tan, K.V. ; Tenditnaya, A. ; Vojnovic, B. ; Gorpas, D. ; Quante, M. ; Vallis, K.A.

Targeting c-MET for endoscopic detection of dysplastic lesions within Barrett's esophagus using EMI-137 fluorescence imaging.

Kriukova, E. ; Mazurenka, M. ; Marcazzan, S. ; Glasl, S. ; Quante, M. ; Saur, D. ; Tschurtschenthaler, M. ; Puppels, G.J. ; Gorpas, D. ; Ntziachristos, V.

Hybrid Raman and partial wave spectroscopy microscope for the characterization of molecular and structural alterations in tissue.

Rauschendorfer, P. ; Lenz, T. ; Nicol, P. ; Wild, L. ; Beele, A. ; Sabic, E. ; Klosterman, G.R. ; Laugwitz, K.L. ; Jaffer, F.A. ; Gorpas, D. ; Joner, M. ; Ntziachristos, V.

Intravascular ICG-enhanced NIRF-IVUS imaging to assess progressive atherosclerotic lesions in excised human coronary arteries.

Tenditnaya, A. ; Gabriels, R.Y. ; Hooghiemstra, W.T.R. ; Klemm, U. ; Nagengast, W.B. ; Ntziachristos, V. ; Gorpas, D.

Performance assessment and quality control of fluorescence molecular endoscopy with a multi-parametric rigid standard.

Marcazzan, S. ; Braz Carvalho, M.J. ; Nguyen, N.T. ; Strangmann, J. ; Slotta-Huspenina, J. ; Tenditnaya, A. ; Tschurtschenthaler, M. ; Rieder, J. ; Proaño-Vasco, A. ; Ntziachristos, V. ; Steiger, K. ; Gorpas, D. ; Quante, M. ; Kossatz, S.

PARP1-targeted fluorescence molecular endoscopy as novel tool for early detection of esophageal dysplasia and adenocarcinoma.

Gabriels, R.Y. ; van Heijst, L.E. ; Hooghiemstra, W.T.R. ; van der Waaij, A.M. ; Kats-Ugurlu, G. ; Karrenbeld, A. ; Robinson, D.J. ; Tenditnaya, A. ; Ntziachristos, V. ; Gorpas, D. ; Nagengast, W.B.

Detection of early esophageal neoplastic barrett lesions with quantified fluorescence molecular endoscopy using Cetuximab-800CW.

Arias, A. ; Anastasopoulou, M. ; Gorpas, D. ; Ntziachristos, V.

Using reflectometry to minimize the dependence of fluorescence intensity on optical absorption and scattering.

Rauschendorfer, P. ; Wissmeyer, G. ; Jaffer, F.A. ; Gorpas, D. ; Ntziachristos, V.

Accounting for blood attenuation in intravascular near-infrared fluorescence-ultrasound imaging using a fluorophore-coated guidewire.

Sikkenk, D.J. ; Sterkenburg, A.J. ; Schmidt, I. ; Gorpas, D. ; Nagengast, W.B. ; Consten, E.C.J.

Detection of tumour-targeted IRDye800CW tracer with commercially available laparoscopic surgical systems.

Seguchi, M. ; Aytekin, A. ; Lenz, T. ; Nicol, P. ; Klosterman, G.R. ; Beele, A. ; Sabic, E. ; Utsch, L. ; Alyaqoob, A. ; Gorpas, D. ; Ntziachristos, V. ; Jaffer, F.A. ; Rauschendorfer, P. ; Joner, M.

Intravascular molecular imaging: Translating pathophysiology of atherosclerosis into human disease conditions.

Gorpas, D. ; Wabnitz, H. ; Pfefer, T.J.

Special section guest editorial: Tissue phantoms to advance biomedical optical systems.

Zhao, X. ; Gabriels, R.Y. ; Hooghiemstra, W.T.R. ; Koller, M. ; Meersma, G.J. ; Buist-Homan, M. ; Visser, L. ; Robinson, D.J. ; Tenditnaya, A. ; Gorpas, D. ; Ntziachristos, V. ; Karrenbeld, A. ; Kats-Ugurlu, G. ; Fehrmann, R.S.N. ; Nagengast, W.B.

Validation of novel molecular imaging targets identified by functional genomic mRNA profiling to detect dysplasia in Barrett's Esophagus.

Sterkenburg, A.J. ; Hooghiemstra, W.T.R. ; Schmidt, I. ; Ntziachristos, V. ; Nagengast, W.B. ; Gorpas, D.

Standardization and implementation of fluorescence molecular endoscopy in the clinic.

Kyrkou, S.G. ; Vrettos, E.I. ; Gorpas, D. ; Crook, T. ; Syed, N. ; Tzakos, A.G.

Design principles governing the development of theranostic anticancer agents and their nanoformulations with photoacoustic properties.

Marcazzan, S. ; Braz Carvalho, M.J. ; Konrad, M. ; Strangmann, J. ; Tenditnaya, A. ; Baumeister, T. ; Schmid, R.M. ; Wester, H.J. ; Ntziachristos, V. ; Gorpas, D. ; Wang, T.C. ; Schottelius, M. ; Quante, M.

CXCR4 peptide-based fluorescence endoscopy in a mouse model of Barrett's esophagus.

Gorpas, D. ; Ntziachristos, V. ; Tian, J.

Principles and practice of intraoperative fluorescence imaging.

Liu, N. ; O'Connor, P. ; Gujrati, V. ; Gorpas, D. ; Glasl, S. ; Blutke, A. ; Walch, A.K. ; Kleigrewe, K. ; Sattler, M. ; Plettenburg, O. ; Ntziachristos, V.

Facile synthesis of a croconaine-based nanoformulation for optoacoustic imaging and photothermal therapy.

Fang, H.Y. ; Stangl, S. ; Marcazzan, S. ; Carvalho, M.J.B. ; Baumeister, T. ; Anand, A. ; Strangmann, J. ; Huspenina, J.S. ; Wang, T.C. ; Schmid, R.M. ; Feith, M. ; Friess, H. ; Ntziachristos, V. ; Multhoff, G. ; Gorpas, D. ; Quante, M.

Targeted Hsp70 fluorescence molecular endoscopy detects dysplasia in Barrett's esophagus.

Liu, N. ; O'Connor, P. ; Gujrati, V. ; Gorpas, D. ; Glasl, S. ; Blutke, A. ; Walch, A.K. ; Kleigrewe, K. ; Sattler, M. ; Plettenburg, O. ; Ntziachristos, V.

Facile synthesis of a croconaine-based nanoformulation for optoacoustic imaging and photothermal therapy.

Varasteh, Z. ; De Rose, F. ; Mohanta, S. ; Li, Y. ; Zhang, X. ; Miritsch, B. ; Scafetta, G. ; Yin, C. ; Sager, H.B. ; Glasl, S. ; Gorpas, D. ; Habenicht, A.J.R. ; Ntziachristos, V. ; Weber, W.A. ; Bartolazzi, A. ; Schwaiger, M. ; D'Alessandria, C.

Imaging atherosclerotic plaques by targeting Galectin-3 and activated macrophages using (⁸⁹Zr)-DFO- Galectin3-F(ab')₂ mAb.

Yim, J.J. ; Harmsen, S. ; Flisikowski, K. ; Flisikowska, T. ; Namkoong, H. ; Garland, M. ; van den Berg, N.S. ; Vilches-Moure, J.G. ; Schnieke, A. ; Saur, D. ; Glasl, S. ; Gorpas, D. ; Habtezion, A. ; Ntziachristos, V. ; Contag, C.H. ; Gambhir, S.S. ; Bogyo, M. ; Rogalla, S.

A protease-activated, near-infrared fluorescent probe for early endoscopic detection of premalignant gastrointestinal lesions.

Gianani, I. ; Suprano, A. ; Giordani, T. ; Spagnolo, N. ; Sciarrino, F. ; Gorpas, D. ; Ntziachristos, V. ; Pinker, K. ; Biton, N. ; Kupferman, J. ; Arnon, S.

Transmission of vector vortex beams in dispersive media.

Suprano, A. ; Giordani, T. ; Gianani, I. ; Spagnolo, N. ; Pinker, K. ; Kupferman, J. ; Arnon, S. ; Klemm, U. ; Gorpas, D. ; Ntziachristos, V. ; Sciarrino, F.

Propagation of structured light through tissue-mimicking phantoms.

Ruiz, A.J. ; Wu, M. ; LaRochelle, E.P.M. ; Gorpas, D. ; Ntziachristos, V. ; Pfefer, T.J. ; Pogue, B.W.

Indocyanine green matching phantom for fluorescence-guided surgery imaging system characterization and performance assessment.

Gorpas, D. ; Koch, M. ; Anastasopoulou, M. ; Bozhko, D. ; Klemm, U. ; Nieberler, M. ; Ntziachristos, V.

Multi-parametric standardization of fluorescence imaging systems based on a composite phantom.

Anastasopoulou, M. ; Gorpas, D. ; Koch, M. ; Liapis, E. ; Glasl, S. ; Klemm, U. ; Karlas, A. ; Lasser, T. ; Ntziachristos, V.

Fluorescence imaging reversion using spatially variant deconvolution.

Rogalla, S. ; Flisikowski, K. ; Gorpas, D. ; Mayer, A.T. ; Flisikowska, T. ; Mandella, M.J. ; Ma, X. ; Casey, K.M. ; Felt, S.A. ; Saur, D. ; Ntziachristos, V. ; Schnieke, A. ; Contag, C.H. ; Gambhir, S.S. ; Harmsen, S.

Biodegradable fluorescent nanoparticles for endoscopic detection of colorectal carcinogenesis.

Gorpas, D. ; Anastasopoulou, M. ; Koch, M. ; Klemm, U. ; Nieberler, M. ; Ntziachristos, V.

Standardization phantom for intra-operative fluorescence molecular imaging.

Wang, H. ; Willershäuser, M. ; Karlas, A. ; Gorpas, D. ; Reber, J. ; Ntziachristos, V. ; Maurer, S. ; Fromme, T. ; Li, Y. ; Klingenspor, M.

A dual Ucp1 reporter mouse model for imaging and quantitation of brown and brite fat recruitment.

Napp, J. ; Markus, M.A. ; Heck, J.G. ; Dullin, C. ; Moebius, W. ; Gorpas, D. ; Feldmann, C. ; Alves, F.

Therapeutic fluorescent hybrid nanoparticles for traceable delivery of glucocorticoids to inflammatory sites.

Schottelius, M. ; Wurzer, A. ; Wissmiller, K. ; Beck, R. ; Koch, M. ; Gorpas, D. ; Notni, J. ; Buckle, T. ; van Oosterom, M.N. ; Steiger, K. ; Ntziachristos, V. ; Schwaiger, M. ; van Leeuwen, F.W.B. ; Wester, H.J.

Synthesis and preclinical characterization of the PSMA-targeted hybrid tracer PSMA-I&F for nuclear and fluorescence imaging of prostate cancer.

Gorpas, D. ; Koch, M. ; Anastasopoulou, M. ; Klemm, U. ; Ntziachristos, V.

Phantom and methodology for fluorescence molecular imaging systems benchmarking.

Gorpas, D. ; Davari, P. ; Bec, J. ; Fung, M.A. ; Marcu, L. ; Farwell, D.G. ; Fazel, N.

Time-resolved fluorescence spectroscopy for the diagnosis of oral lichen planus.

Bozhko, D. ; Karlas, A. ; Gorpas, D. ; Ntziachristos, V.

Optoacoustic sensing of hematocrit to improve the accuracy of hybrid fluorescence-ultrasound intravascular imaging.

Phipps, J.E. ; Gorpas, D. ; Unger, J. ; Darrow, M. ; Bold, R.J. ; Marcu, L.

Automated detection of breast cancer in resected specimens with fluorescence lifetime imaging.

Bec, J. ; Phipps, J.E. ; Gorpas, D. ; Ma, D. ; Fatakdawala, H. ; Margulies, K.B. ; Southard, J.A. ; Marcu, L.

In vivo label-free structural and biochemical imaging of coronary arteries using an integrated ultrasound and multispectral fluorescence lifetime catheter system.

Fereidouni, F. ; Gorpas, D. ; Ma, D. ; Fatakdawala, H. ; Marcu, L.