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Research Group/Lab: Stage AAP

To complement clinical and basic research units, the Research Unit Analytical Pathology at the Helmholtz Zentrum München scientifically develops translational research on diseases that manifest in tissues.

For example, the Research Unit Analytical Pathology explores the translation of in-vitro models or animal models into applications for humans. These could be laboratory methods that open up new avenues for diagnosis or attempts to explain why a certain disease develops under certain conditions. The primary research objectives are the search for diagnostic/theragnostic biomarkers and new target structures for treatment, as well as histopharmacological analysis of active substances in healthy, predisposed or diseased tissues in a future personalized medication.

The Research Unit Analytical Pathology links basic research and diagnostic application, and translates the findings from experimental and molecular pathology into procedures for disease classification and predictive diagnostics on tissue.

Microscopic imaging methods are key technologies for the molecular analysis of healthy, predisposed and pathologically altered tissues and cells. The Research Unit Analytical Pathology focuses its cutting edge infrastructure and expertise in order to research diseases that manifest in tissues. All current platform technologies combine microscopic imaging methods with the analysis of individual molecules or complex molecular signatures. The starting point is always the classic morphological evaluation of the tissues and cells to be analyzed, a step that is necessary for an exact categorization of molecular alterations at the level of individual cells or cell organelles.

  • Application and development of microscopic imaging for combined morphological and molecular analysis of tissues and cells
  • Development of imaging methods for active agent analysis in tissues
  • Image analysis platform for microscope image data

 

 

 

 

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Research Group/Lab: About our Unit| Option A

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The Research Unit Analytical Pathology (AAP) carries out scientific development, as a complement to research units with a clinical and fundamental orientation of translational research on diseases that occur in tissue.

Body text: Explanatory body text for this content area. You can find more information about the tone of voice guidelines in the “Text Guide” section...

AAP is involved in the translation of (for example) in-vitro models or animal models to application in humans. AAP thus links basic research with diagnostic application, subsequently translating the findings of experimental and molecular pathology into procedures for the classification of diseases and predictive diagnostics dealing with tissue.

 

 

 

 

Research Group/Lab: About our … | Option B

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Read-in text: The read-in text gives the users an initial overview of what the article is about. Ideally no more than three lines (max. approx. 180 characters)...

The Research Unit Analytical Pathology (AAP) carries out scientific development, as a complement to research units with a clinical and fundamental orientation of translational research on diseases that occur in tissue.

Body text: Explanatory body text for this content area. You can find more information about the tone of voice guidelines in the “Text Guide” section...

AAP is involved in the translation of (for example) in-vitro models or animal models to application in humans. AAP thus links basic research with diagnostic application, subsequently translating the findings of experimental and molecular pathology into procedures for the classification of diseases and predictive diagnostics dealing with tissue.

 

 

 

 

Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet.

 

Lorem ipsum dolor sit amet, consetetur sadipscing elitr, Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet.

Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet.

 

Read-in text: The read-in text gives the users an initial overview of what the article is about. Ideally no more than three lines (max. approx. 180 characters)...

The Research Unit Analytical Pathology (AAP) carries out scientific development, as a complement to research units with a clinical and fundamental orientation of translational research on diseases that occur in tissue.

Body text: Explanatory body text for this content area. You can find more information about the tone of voice guidelines in the “Text Guide” section...

AAP is involved in the translation of (for example) in-vitro models or animal models to application in humans. AAP thus links basic research with diagnostic application, subsequently translating the findings of experimental and molecular pathology into procedures for the classification of diseases and predictive diagnostics dealing with tissue.

 

 

 

 

Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet.

 

Lorem ipsum dolor sit amet, consetetur sadipscing elitr, Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet.

Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet.

 

Research Group/Lab: Scientists at AAP| Option A

Prof. Dr. med. Axel Karl Walch

Head

Dr. Michaela Aichler

Scientist

Ulrike Buchholz

MTLA

Claudia-Mareike Pflüger

MTLA
Portrait Andreas Voss

Dipl.-Ing. Andreas Voss

Engineer

Dr. Na Sun

Postdoc

Sükriye Koccayir

Service Assistant

Jun Wang

PhD Student

Qian Wang

PhD student

Chaoyang Zhang

PhD Student

Research Group/Lab: Scientists at AAP| Option B

Prof. Dr. med. Axel Karl Walch

Head

Dr. Michaela Aichler

Scientist

Ulrike Buchholz

MTLA

Claudia-Mareike Pflüger

MTLA
Portrait Andreas Voss

Dipl.-Ing. Andreas Voss

Engineer

Dr. Na Sun

Postdoc

Sükriye Koccayir

Service Assistant

Jun Wang

PhD Student

Qian Wang

PhD student

Chaoyang Zhang

PhD Student

Research Area: Our Topics

Diabetes and Obesity

In the field of diabetes research we focus on the morphological and molecular background of the disease in the endocrine pancreas, insulin resistance in peripheral organs and complications which are caused by type 2 diabetes to generate a deeper understanding of the disease which can be used as a new basis for interventional strategies. High-resolution mass spectrometry imaging (MALDI imaging) is used for investigating unsolved questions in diabetes research. The technology makes it possible to examine the distribution of metabolic products (cell metabolites) and many other molecular classes directly in tissue sections without the need of tissue homogenization and thus enables the generation of very high quality and complex data. Data are further processed in highly sophisticated statistical approaches to extract biologically important information. The unique combination of thus generated data with further in depth tissue analytics as electron microscopy or in vitro analytics we use to generate a deeper understanding of the background of diseases. In the field of diabetes research we focus on the morphological and molecular background of the disease in the endocrine pancreas, insulin resistance in peripheral organs and complications which are caused by type 2 diabetes to generate a deeper understanding of the disease which can be used as a new basis for interventional strategies. High-resolution mass spectrometry imaging (MALDI imaging) is used for investigating unsolved questions in diabetes research. The technology makes it possible to examine the distribution of metabolic products (cell metabolites) and many other molecular classes directly in tissue sections without the need of tissue homogenization and thus enables the generation of very high quality and complex data. Data are further processed in highly sophisticated statistical approaches to extract biologically important information. The unique combination of thus generated data with further in depth tissue analytics as electron microscopy or in vitro analytics we use to generate a deeper understanding of the background of diseases.

Endocrine pathology

In situ metabolite MALDI imaging offers enormous clinical potential by enabling the imaging of a largely previously intractable class of biomolecules. Combined with known metabolic pathways, this approach provides a means to image the activities of the pathways in tissues. The endocrine system is a collection of glands that secrete hormones directly into the circulatory system. The MALDI imaging based endocrinology project fills the heuristic gap to visualize the distributions of hormones, metabolites and drugs within tissues. Recently, tissue metabolomic studies have been initiated on adrenal tissues, which have revealed a refined functional structure beyond classical histological anatomy in human adrenal glands (Sun et al. Endocrinology, 2018 PubMed). Future developments will extend the so far existing clinicopathologic application areas by imaging the activities of pathways in tissues, imaging of hormones (steroids, catecholamines), drugs and their metabolites in the cortex and medulla of the adrenal glands and other endocrine organs of tissue from patients and animal models. The aims of these projects are to advance the understanding of the mechanism of endocrine diseases and to improve diagnosis, prognostic and therapeutic decision making.

Cancer and inter-/intratumoral heterogeneity

Understanding tumor heterogeneity presents one of the most important tasks in cancer research towards improving both diagnosis and treatment of patients. Heterogeneity of tumors contributes to treatment failure and disease recurrence, whereas the interaction between tumor cells and the associated stroma poses novel therapeutic opportunities. Intratumoral heterogeneity refers to the fact that cells within a tumor mass can be highly diverse due to an evolving process in cancer development driven by the stepwise accumulation of molecular changes and clonal selection, while intertumoral heterogeneity describes molecular alterations in several (metastatic) tumors present within or between patients. In malignant cells alteration in molecular features and clonal selection constantly takes place and provides either a selective advantage in function such as proliferation and survival, proceed neutral without any effects, or has negative cell damaging effects. Thereby, the fitness of a neoplastic cell is affected by the interactions with itself and other cells in its microenvironment competing with one another for the available resources. Tumor heterogeneity has mainly been described at a genetic, chromosomal, or transcriptional level. The approaches used to analyze tumor heterogeneity are mostly targeted either focusing on the distribution of a single molecule in tissues such as proteins by using immunohistochemistry, or provide detailed molecular insight in tumor subpopulations by the targeted selection of tumor subpopulations. However, selection of representative tumor areas leads only to an average picture which describes the most dominant clone and therefore underestimates the extent and pattern of clonal heterogeneity. MALDI MSI has demonstrated its suitability to study tumor heterogeneity in its native microenvironment.

Research Group/Lab: Publications | Option A

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M. Mustermann, P. Mustermann, C. Mustermann

M. Mustermann, P. Mustermann, C. Mustermann

Research Group/Lab: Publications | Option B

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M. Mustermann, P. Mustermann, C. Mustermann

M. Mustermann, P. Mustermann, C. Mustermann