Skip to main content
INSPECT_alternative_credit_Barth_van_Rossum
©Barth-Jan van Rossum

Non-invasive monitoring of non-coding RNA | how to encode proteins from intronic information?

ISBM,

We introduce a new gene reporter system (INSPECT) that encode reporter or effector proteins from intronic information.

We introduce a new gene reporter system (INSPECT) that can dynamically monitor the expression of long non-coding RNAs (lncRNAs) without modifying the mature target RNA sequence. This capability is achieved by embedding INSPECT into an intron of the gene of interest, such that its post-transcriptional excision during RNA-splicing results in a synthetic transcript, which is exported out of the nucleus and translated into a reporter or effector protein of choice.

https://www.helmholtz-munich.de/en/newsroom/news-all/artikel/molecular-monitoring-of-rna-regulation

https://doi.org/10.1038/s41556-022-00998-6