Proteins come in many flavors | high-throughput tracking of specific protein isoforms
We have generated a suite of gene reporters, EXSISERS, that self-excise themselves out of the native amino-acid chain of a protein of interest to report on the translation of specific protein isoforms from alternatively spliced mRNA.
We have used EXSISERS for ratiometric monitoring of isoforms of the Tau protein.
Please find a detailed protocol for EXSISERS here,
and the genetic constructs deposited here: