Based on a translational research approach with numerous clinical cooperation partners on campus and abroad, we employ biochemical and molecular biology technologies in mouse disease models, cell culture systems and human patient samples.

1. Mouse disease models

Obese mouse (dkfz)
  • Genetic mouse models, CreLoxP-mediated mutagenesis
  • Metabolic mouse models, dietary/hormonal intervention
  • Tumor implantation models/ chemically-induced carcinogenesis
  • Acute genetic manipulation via adeno- and AAV systems
  • Metabolic phenotyping, indirect calorimetry, MRI
  • High Throughput Gene Expression analysis
  • In vivo protein-DNA interaction studies
  • Tracer studies: Quantification of physiological processes

2. Cell culture and in vitro systems

Hepatocytes (Zeigerer/IDC)
  • Primary cell culture, hepatocytes and adipocytes
  • Tumor cell lines
  • Protein-protein interaction studies
  • Functional gene activity studies
  • Tracer studies: Quantification of physiological processes

3. Human patient samples

Gene activity (Scheideler/IDC)
  • Gene expression analysis
  • Functional metabolic pathway studies
  • DNA recruitment assays
  • Human bio banks