Cloning

Cloning protocols

Preparation of oligo solutions

PCR experiments

Digest of insert DNA

Digest and dephosphorylation of vector DNA

Ligation of DNA fragments

Preparation of chemically competent cells

Transformation of plasmid DNA into chemically competent cells

Ligase independent cloning (LIC)

Ligase independent cloning (LIC) using pETM-13/LIC

  • Creating a construct with a C-terminal His6-tag

Ligase independent cloning (LIC) protocols