Cloning
Cloning protocols
Preparation of oligo solutions
Digest of insert DNA
Digest and dephosphorylation of vector DNA
Ligation of DNA fragments
Preparation of chemically competent cells
Transformation of plasmid DNA into chemically competent cells
Ligase independent cloning (LIC)
Ligase independent cloning (LIC) using pETM-13/LIC
- Creating a construct with a C-terminal His6-tag
Ligase independent cloning (LIC) protocols
- Ligase independent cloning (LIC)
- Ligase independent cloning (LIC) using pETM-13/LIC
Creating a construct with a C-terminal His6-tag
