Bacterial expression vectors

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Escherichia coli expression vectors

Vector	Vector map	Promoter	Antibiotic resistance	Tag(s)	Protease cleavage site	Expression host	Origin of replication	Source	Description
pACYCDuet-1	map	T7/lac	Cam	N-His		BL21 (DE3)	p15A	Novagen	expression vector; co-expression of 2 target genes
pACYC-11	map	T7/lac	Cam	N-His	TEV	BL21 (DE3)	p15A	EMBL-AG	expression vector
pACYC-13	map	T7/lac	Cam	C-His	TEV	BL21 (DE3)	p15A	EMBL-AG	expression vector
pACYC-31	map	T7/lac	Cam	N-His N-GST	TEV	BL21 (DE3)	p15A	EMBL-AG	expression vector
pASK75		tet	Amp	Strep		BL21		Biometra	expression vector
pBAD/His A	map	araBAD	Amp	N-His	EK	Top10 LMG194	pUC	Invitrogen	expression vector
pBAD/His B	map	araBAD	Amp	N-His	EK	Top10 LMG194	pUC	Invitrogen	expression vector
pBAD/His C	map	araBAD	Amp	N-His	EK	Top10 LMG194	pUC	Invitrogen	expression vector
pBAD/mcs	map	araBAD	Amp	N-His		Top10 LMG194	pUC	EMBL-AG	expression vector
pBADM-11	map	araBAD	Amp	N-His	TEV	Top10 LMG194	pUC	EMBL-AG	expression vector
pBADM-20	map	araBAD	Amp	N-TrxA N-His	TEV	Top10 LMG194	pUC	EMBL-AG	expression vector
pBADM-20(+)	map	araBAD	Amp	N-TrxA N-His	TEV	Top10 LMG194	pUC	EMBL-AG	expression vector
pBADM-30	map	araBAD	Amp	N-His N-GST	TEV	Top10 LMG194	pUC	EMBL-AG	expression vector
pBADM-30(+)	map	araBAD	Amp	N-His N-GST	TEV	Top10 LMG194	pUC	EMBL-AG	expression vector
pBADM-40(+)	map	araBAD	Amp	N-MBP	TEV	Top10 LMG194	pUC	EMBL-AG	expression vector
pBADM-41(+)	map	araBAD	Amp	N-His N-MBP	TEV	Top10 LMG194	pUC	EMBL-AG	expression vector
pBADM-52	map	araBAD	Amp	N-leaderless-DsbA N-His	TEV	Top10 LMG194	pUC	EMBL-AG	expression vector
pBADM-52(+)	map	araBAD	Amp	N-leaderless-DsbA N-His	TEV	Top10 LMG194	pUC	EMBL-AG	expression vector
pBADM-60	map	araBAD	Amp	N-NusA N-His	TEV	Top10 LMG194	pUC	EMBL-AG	expression vector
pBADM-60(+)	map	araBAD	Amp	N-NusA N-His	TEV	Top10 LMG194	pUC	EMBL-AG	expression vector
pBAT4	map	T7	Amp			BL21 (DE3)	pUC	EMBL-MH	expression vector
pBAT5	map	T7	Amp			BL21 (DE3)	pUC	EMBL-MH	expression vector
pCal-n	map	T7/lac	Amp	CBP	thrombin	BL21 (DE3)	ColE1	Stratagene	expression vector
pCDF-1b	map	T7/lac	Spec/Strep	N-His	EK	BL21 (DE3)	CDF	Novagen	expression vector
pCDF-11	map	T7/lac	Spec/Strep	N-His	TEV	BL21 (DE3)	CDF	EMBL-AG	expression vector
pCDF-13	map	T7/lac	Spec/Strep	C-His	TEV	BL21 (DE3)	CDF	EMBL-AG	expression vector
pCDFDuet-1	map	T7/lac	Spec/Strep	N-His		BL21 (DE3)	CDF	Novagen	expression vector; co-expression of 2 target genes
pET-3a	map	T7	Amp			BL21 (DE3)	pBR322	Novagen	expression vector
pET-3b	map	T7	Amp			BL21 (DE3)	pBR322	Novagen	expression vector
pET-3c	map	T7	Amp			BL21 (DE3)	pBR322	Novagen	expression vector
pET-3d	map	T7	Amp			BL21 (DE3)	pBR322	Novagen	expression vector
pET-12a	map	T7	Amp	leader sequence		BL21 (DE3)	pBR322	Novagen	expression vector
pET-14b	map	T7	Amp	N-His	Thrombin	BL21 (DE3)	pBR322	Novagen	expression vector
pET-15b	map	T7/lac	Amp	N-His	Thrombin	BL21 (DE3)	pBR322	Novagen	expression vector
pET-16b	map	T7/lac	Amp	N-His10	Factor Xa	BL21 (DE3)	pBR322	Novagen	expression vector
pET-19b	map	T7/lac	Amp	N-His	EK	BL21 (DE3)	pBR322	Novagen	expression vector
pET-20b(+)	map	T7	Amp	leader sequence C-His		BL21 (DE3)	pBR322	Novagen	expression vector for secreted proteins
pET-21d(+)	map	T7/lac	Amp	C-His		BL21 (DE3)	pBR322	Novagen	expression vector
pET-22b(+)	map	T7/lac	Amp	leader sequence C-His		BL21 (DE3)	pBR322	Novagen	expression vector for secreted proteins
pET-24a(+)	map	T7/lac	Kan	C-His		BL21 (DE3)	pBR322	Novagen	expression vector
pET-24d(+)	map	T7/lac	Kan	C-His		BL21 (DE3)	pBR322	Novagen	expression vector
pET-28a(+)	map	T7/lac	Kan	N-His C-His	Thrombin	BL21 (DE3)	pBR322	Novagen	expression vector
pET-28c(+)	map	T7/lac	Kan	N-His C-His	Thrombin	BL21 (DE3)	pBR322	Novagen	expression vector
pET-32a(+)	map	T7/lac	Amp	N-TrxA N-His C-His	Thrombin EK	AD494 (DE3) Origami (DE3)	pBR322	Novagen	expression vector for disulfite bond containing proteins
pET-32b(+)	map	T7/lac	Amp	N-TrxA N-His C-His	Thrombin EK	AD494 (DE3) Origami (DE3)	pBR322	Novagen	expression vector for disulfite bond containing proteins
pET-32c(+)	map	T7/lac	Amp	N-TrxA N-His C-His	Thrombin EK	AD494 (DE3) Origami (DE3)	pBR322	Novagen	expression vector for disulfite bond containing proteins
pET-39b(+)	map	T7/lac	Kan	N-DsbA C-His	Thrombin EK	BL21 (DE3)	pBR322	Novagen	expression vector for secreted proteins
pET-40b(+)	map	T7/lac	Kan	N-DsbC N-His C-His	Thrombin EK	BL21 (DE3)	pBR322	Novagen	expression vector for secreted proteins
pETcoco-1	map	T7/lac	Cam	N-His	EK	BL21 (DE3)	oriS oriV	Novagen	expression vector; allows single and medium vector copy number
pETDuet-1	map	T7/lac	Amp	N-His		BL21 (DE3)	pBR322		expression vector; co-expression of 2 target genes
pET DsbA-1b	map	T7/lac	Kan	N-LL-DsbA N-His	TEV	BL21 (DE3)	pBR322	EMBL-GS	modified pET-24d, leaderless DsbA for cytoplasmic expression
pET DsbC-1b	map	T7/lac	Kan	N-LL-DsbC N-His	TEV	BL21 (DE3)	pBR322	EMBL-GS	modified pET-24d, leaderless DsbC for cytoplasmic expression
pET GB-1a	map	T7/lac	Kan	N-His N-GB1	TEV	BL21 (DE3)	pBR322	EMBL-GS	modified pET-24d
pET GB-1b	map	T7/lac	Kan	N-GB1 N-His	TEV	BL21 (DE3)	pBR322	EMBL-GS	modified pET-24d
pET GST-1a	map	T7/lac	Kan	N-His N-GST	TEV	BL21 (DE3)	pBR322	EMBL-GS	modified pET-24d
pET GST-1b	map	T7/lac	Kan	N-GST N-His	TEV	BL21 (DE3)	pBR322	EMBL-GS	modified pET-24d
pET MBP-1a	map	T7/lac	Kan	N-His N-MBP	TEV	BL21 (DE3)	pBR322	EMBL-GS	modified pET-24d
pET MBP-1b	map	T7/lac	Kan	N-MBP N-His	TEV	BL21 (DE3)	pBR322	EMBL-GS	modified pET-24d
pET NusA-1a	map	T7/lac	Kan	N-His N-NusA	TEV	BL21 (DE3)	pBR322	EMBL-GS	modified pET-24d
pET NusA-1b	map	T7/lac	Kan	N-NusA N-His	TEV	BL21 (DE3)	pBR322	EMBL-GS	modified pET-24d
pET TrxA-1a	map	T7/lac	Kan	N-His N-TrxA	TEV	BL21 (DE3)	pBR322	EMBL-GS	modified pET-24d
pET TrxA-1b	map	T7/lac	Kan	N-TrxA N-His	TEV	BL21 (DE3)	pBR322	EMBL-GS	modified pET-24d
pET Z fl-1b	map	T7/lac	Kan	N-Z-tag (fl) N-His	TEV	BL21 (DE3)	pBR322	EMBL-GS	modified pET-24d
pET Z2-1a	map	T7/lac	Kan	N-His N-Z2	TEV	BL21 (DE3)	pBR322	EMBL-GS	modified pET-24d
pET Z2-1b	map	T7/lac	Kan	N-Z2 N-His	TEV	BL21 (DE3)	pBR322	EMBL-GS	modified pET-24d
pETM-10	map	T7/lac	Kan	N-His C-His		BL21 (DE3)	pBR322	EMBL-GS	modified pET-24d
pETM-11	map	T7/lac	Kan	N-His	TEV	BL21 (DE3)	pBR322	EMBL-GS	modified pET-24d
pETM-12	map	T7/lac	Kan	N-His	Thrombin	BL21 (DE3)	pBR322	EMBL-GS	modified pET-24d
pETM-13	map	T7/lac	Kan	C-His		BL21 (DE3)	pBR322	EMBL-GS	modified pET-24d
pETM-14	map	T7/lac	Kan	N-His	3C	BL21 (DE3)	pBR322	EMBL-AG	modified pET-24d
pETM-20	map	T7/lac	Amp	N-TrxA N-His	TEV	BL21 (DE3)	pBR322	EMBL-GS	modified pET-32
pETM-21		T7/lac	Amp	N-TrxA N-His	Thrombin	BL21 (DE3)	pBR322	EMBL-GS	modified pET-32
pETM-22	map	T7/lac	Kan	N-TrxA N-His	3C	BL21 (DE3)	pBR322	EMBL-AG	modified pET-24d
pETM-30	map	T7/lac	Kan	N-His N-GST	TEV	BL21 (DE3)	pBR322	EMBL-GS	modified pET-24d
pETM-33	map	T7/lac	Kan	N-His N-GST	3C	BL21 (DE3)	pBR322	EMBL-AG	modified pET-24d
pETM-40	map	T7/lac	Kan	N-MBP	TEV	BL21 (DE3)	pBR322	EMBL-GS	modified pET-24d
pETM-41	map	T7	Kan	N-His N-MBP	TEV	BL21 (DE3)	pBR322	EMBL-GS	modified pET-9d
pETM-43	map	T7/lac	Kan	N-MBP	3C	BL21 (DE3)	pBR322	EMBL-AG	modified pET-24d
pETM-44	map	T7/lac	Kan	N-His N-MBP	3C	BL21 (DE3)	pBR322	EMBL-AG	modified pET-24d
pETM-50	map	T7/lac	Kan	N-DsbA N-His	TEV	BL21 (DE3)	pBR322	EMBL-GS	modified pET-24d; vector for periplasmic expression
pETM-51		T7/lac	Kan	N-DsbA N-His	Thrombin	BL21 (DE3)	pBR322	EMBL-GS	modified pET-24d; vector for periplasmic expression
pETM-52	map	T7/lac	Kan	N-leaderless-DsbA N-His	TEV	BL21 (DE3)	pBR322	EMBL-GS	modified pET-24d, leaderless DsbA for cytoplasmic expression
pETM-54	map	T7/lac	Kan	N-DsbA N-His	3C	BL21 (DE3)	pBR322	EMBL-AG	modified pET-24d
pETM-55	map	T7/lac	Kan	N-LL-DsbA N-His	3C	BL21 (DE3)	pBR322	EMBL-AG	modified pET-24d; leaderless DsbA for cytoplasmic expression
pETM-60	map	T7/lac	Kan	N-NusA N-His	TEV	BL21 (DE3)	pBR322	EMBL-GS	modified pET-24d
pETM-66	map	T7/lac	Kan	N-NusA N-His	3C	BL21 (DE3)	pBR322	EMBL-AG	modified pET-24d
pETM-70	map	T7/lac	Kan	N-CBP	TEV	BL21 (DE3)	pBR322	EMBL-AG	modified pET-24d
pETM-80	map	T7/lac	Kan	N-DsbC N-His	TEV	BL21 (DE3)	pBR322	EMBL-GS	modified pET-24d, vector for periplasmic expression
pETM-82	map	T7	Kan	N-LL-DsbC N-His	TEV	BL21 (DE3)	pBR322	EMBL-GS	modified pET-9d; leaderless DsbC for cytoplasmic expression
pETM-90	map	T7/lac	Amp	N-FTR	TEV	BL21 (DE3)	pBR322	EMBL-AM	expression vector
pGAT	map	T7	Amp	N-His N-GST	Thrombin		pUC	EMBL-MH	expression vector
pGAT2	map	T7	Amp	N-His N-GST	Thrombin	BL21	pUC	EMBL-MH	expression vector
pGEX-3X	map	tac	Amp	N-GST	Factor Xa	BL21	pBR322	GE-Healthcare	expression vector
pGEX-4T-1	map	tac	Amp	N-GST	Thrombin	BL21	pBR322	GE-Healthcare	expression vector
pGEX-4T-2	map	tac	Amp	N-GST	Thrombin	BL21	pBR322	GE-Healthcare	expression vector
pGEX-4T-3	map	tac	Amp	N-GST	Thrombin	BL21	pBR322	GE-Healthcare	expression vector
pGEX-5X-1	map	tac	Amp	N-GST	Factor Xa	BL21	pBR322	GE-Healthcare	expression vector
pGEX-5X-2	map	tac	Amp	N-GST	Factor Xa	BL21	pBR322	GE-Healthcare	expression vector
pGEX-5X-3	map	tac	Amp	N-GST	Factor Xa	BL21	pBR322	GE-Healthcare	expression vector
pGEX-6P-1	map	tac	Amp	N-GST	3C	BL21	pBR322	GE-Healthcare	expression vector
pGEX-6P-2	map	tac	Amp	N-GST	3C	BL21	pBR322	GE-Healthcare	expression vector
pGEX-6P-3	map	tac	Amp	N-GST	3C	BL21	pBR322	GE-Healthcare	expression vector
pGEXM7	map	tac	AMP	N-GST		BL21	pBR322		expression vector
pHAT	map	T7/lac	Amp	N-His		BL21 (DE3)	pUC	EMBL-MH	expression vector
pHAT2	map	T7/lac	Amp	N-His		BL21 (DE3)	pUC	EMBL-MH	expression vector
pKK223-3	map	tac	Amp				GE-Healthcare	expression vector
pKK233-2		trc	Amp					GE-Healthcare	expression vector
pMal-c2	map	tac	Amp	N-MBP	factor Xa	TB1	pBR322	NEB	expression vector
pMal-p2	map	tac	Amp	leader sequence N-MBP	factor Xa	TB1	pBR322	NEB	vector for expression in periplasm
pProEx HTa	map	trc	Amp	N-His	TEV	BL21		Life Technologies	expression vector
pProEx HTb	map	trc	Amp	N-His	TEV	BL21		Life Technologies	expression vector
pProEx HTc	map	trc	Amp	N-His	TEV	BL21		Life Technologies	expression vector
pQE-16	map	T5/lac	Amp	N-DHFR C-His		M15 [pREP4]	ColE1	Qiagen	expression vector
pQE-30	map	T5/lac	Amp	N-His		M15 [pREP4]	ColE1	Qiagen	expression vector
pQE-31	map	T5/lac	Amp	N-His		M15 [pREP4]	ColE1	Qiagen	expression vector
pQE-32	map	T5/lac	Amp	N-His		M15 [pREP4]	ColE1	Qiagen	expression vector
pQE-60	map	T5/lac	Amp	C-His		M15 [pREP4]	ColE1	Qiagen	expression vector
pQE-70	map	T5/lac	Amp	C-His		M15 [pREP4]	ColE1	Qiagen	expression vector
pQE-80L	map	T5/lac	Amp	N-His		M15	ColE1	Qiagen	expression vector also expressing LacI
pQE-81L	map	T5/lac	Amp	N-His		M15	ColE1	Qiagen	expression vector also expressing LacI
pQE-82L	map	T5/lac	Amp	N-His		M15	ColE1	Qiagen	expression vector also expressing LacI
pRSET A	map	T7	Amp	N-His	EK	BL21 (DE3) pLysS	ColE1	Invitrogen	expression vector
pRSET B	map	T7	Amp	N-His	EK	BL21 (DE3) pLysS	ColE1	Invitrogen	expression vector
pRSET C	map	T7	Amp	N-His	EK	BL21 (DE3) pLysS	ColE1	Invitrogen	expression vector
pRSF-1b	map	T7/lac	Kan	N-His	TEV	BL21 (DE3)	RSF1030	Novagen	expression vector
pRSF-11	map	T7/lac	Kan	N-His	TEV	BL21 (DE3)	RSF1030	EMBL-AG	expression vector
pRSF-13	map	T7/lac	Kan	C-His	TEV	BL21 (DE3)	RSF1030	EMBL-AG	expression vector
pRSFDuet-1	map	T7/lac	Kan	N-His		BL21 (DE3)	RSF1030	Novagen	expression vector; co-expression of 2 target genes
pTrcHis2 A	map	trc/lac	Amp	C-His		BL21	pUC	Invitrogen	expression vector
pTrcHis2 B	map	trc/lac	Amp	C-His		BL21	pUC	Invitrogen	expression vector
pTrcHis2 C	map	trc/lac	Amp	C-His		BL21	pUC	Invitrogen	expression vector
pTrcHis2/LacZ	map	trc/lac	Amp	C-His		BL21	pUC	Invitrogen	expression vector
pZA31-Luc	map	PLtetO-1	Cam			DH5αZ1	p15A	UniHD-HB	expression vector; contains luciferase gene
pZE12-Luc	map	PLlacO-1	Amp			DH5αZ1	ColE1	UniHD-HB	expression vector; contains luciferase gene
pZE21-MCS-1	map	PLtetO-1	Kan			DH5αZ1	ColE1	UniHD-HB	high copy number expression vector
*pZS24-MCS-1**	map	Plac/ara-1	Kan			DH5αZ1	pSC*101	UniHD-HB	low copy number expression vector

Source

EMBL-AG = EMBL-made vector by Arie Geerlof
EMBL-AM = EMBL-made vector by Ario de Marco/Elisabetta Cassata
EMBL-GS = EMBL-made vector by Gunter Stier
EMBL-MH = EMBL-made vector by Marko Hyvönen
UniHD-HB = vector made by Prof. Herman Bujard,University of Heidelberg, Germany