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STB -> PEPP -> Materials -> Vector database -> Bacterial cloning and auxiliary vectors

Bacterial cloning and auxiliary vectors

VectorVector mapPromoterAntibiotic resistanceTag(s)Expression hostOrigin of replicationSourceDescription
pACYC 177map Amp
Kan
 DHα5p15ANEBcloning vector
pET-24(+)mapT7/lacKanC-HisBL21 (DE3)pBR322Novagentranscription vector; no ribosome binding site or transcription start codon
pJY1
pLysE-ArgU
 T7
Tc
Cam BL21 (DE3)p15AAffinitiencodes rare ArgU codons
encodes T7 lysosyme (higher concentration than in pJY2)
pJY2
pLysS-ArgU
mapT7
Tc
Cam BL21 (DE3)p15AAffinitiencodes rare ArgU codons
encodes T7 lysosyme
pLysSmapT5/lacCam BL21p15AQiagenencodes T7 lysosyme that blocks leaky expression from T7 promoter by inhibiting T7 RNA polymerase
pPCR-ScriptmapT7
T3
Amp DHα5ColE1Stratagenecloning vector
pPCR-Script eGFPmapT7
T3
Amp DHα5ColE1EMBL-AGcloning vector; containing eGFP stuffer
pRAREmap Cam  p15ANovagenencodes rare codon tRNAs: argU, argW, glyT, ileX, leuW, metT, proL, thrT, thrU and tyrU.
pRARE2map Cam  p15ANovagenencodes rare codon tRNAs: argU, argW, argX, glyT, ileX, leuW, metT, proL, thrT, thrU and tyrU.
pRARELysS  Cam  p15ANovagenencodes T7 lysosyme that blocks leaky expression from T7 promoter by inhibiting T7 RNA polymerase; encodes rare codon tRNAs: argU, argW, glyT, ileX, leuW, metT, proL, thrT, thrU and tyrU.
pREP4mapunknownKan M15p15AQiagenencodes lacI that blocks leaky expression before IPTG induction
pSP73mapSP6Amp DHα5unknownPromegacloning and in vitro transcription vector
pTA PlusmapT7
T3
Amp DHα5ColE1EMBL-AGTA-cloning vector
pUBS ArgU amp  Amp  p15A encodes rare ArgU codons
pUBS ArgU kan  Kan  p15A encodes rare ArgU codons