STB -> PEPP -> Materials -> Vector database -> Bacterial cloning and auxiliary vectors
Bacterial cloning and auxiliary vectors
Vector | Vector map | Promoter | Antibiotic resistance | Tag(s) | Expression host | Origin of replication | Source | Description |
---|---|---|---|---|---|---|---|---|
pACYC 177 | map | Amp Kan | DHα5 | p15A | NEB | cloning vector | ||
pET-24(+) | map | T7/lac | Kan | C-His | BL21 (DE3) | pBR322 | Novagen | transcription vector; no ribosome binding site or transcription start codon |
pJY1 pLysE-ArgU | T7 Tc | Cam | BL21 (DE3) | p15A | Affiniti | encodes rare ArgU codons encodes T7 lysosyme (higher concentration than in pJY2) | ||
pJY2 pLysS-ArgU | map | T7 Tc | Cam | BL21 (DE3) | p15A | Affiniti | encodes rare ArgU codons encodes T7 lysosyme | |
pLysS | map | T5/lac | Cam | BL21 | p15A | Qiagen | encodes T7 lysosyme that blocks leaky expression from T7 promoter by inhibiting T7 RNA polymerase | |
pPCR-Script | map | T7 T3 | Amp | DHα5 | ColE1 | Stratagene | cloning vector | |
pPCR-Script eGFP | map | T7 T3 | Amp | DHα5 | ColE1 | EMBL-AG | cloning vector; containing eGFP stuffer | |
pRARE | map | Cam | p15A | Novagen | encodes rare codon tRNAs: argU, argW, glyT, ileX, leuW, metT, proL, thrT, thrU and tyrU. | |||
pRARE2 | map | Cam | p15A | Novagen | encodes rare codon tRNAs: argU, argW, argX, glyT, ileX, leuW, metT, proL, thrT, thrU and tyrU. | |||
pRARELysS | Cam | p15A | Novagen | encodes T7 lysosyme that blocks leaky expression from T7 promoter by inhibiting T7 RNA polymerase; encodes rare codon tRNAs: argU, argW, glyT, ileX, leuW, metT, proL, thrT, thrU and tyrU. | ||||
pREP4 | map | unknown | Kan | M15 | p15A | Qiagen | encodes lacI that blocks leaky expression before IPTG induction | |
pSP73 | map | SP6 | Amp | DHα5 | unknown | Promega | cloning and in vitro transcription vector | |
pTA Plus | map | T7 T3 | Amp | DHα5 | ColE1 | EMBL-AG | TA-cloning vector | |
pUBS ArgU amp | Amp | p15A | encodes rare ArgU codons | |||||
pUBS ArgU kan | Kan | p15A | encodes rare ArgU codons |