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LIC expression vectors
Bacterial expression vectors for the ligase independent cloning (LIC) system
| Vector | Vector map | Promoter | Antibiotic resistance | Tag(s) | Protease cleavage site | Expression host | Origin of replication | Source | Description |
|---|---|---|---|---|---|---|---|---|---|
| pETM-11/LIC | map | T7/lac | Kan | N-His | TEV | BL21 (DE3) | pBR322 | EMBL-AG | expression vector |
| pETM-11/LIC+ | map | T7/lac | Kan | N-His | TEV | BL21 (DE3) | pBR322 | EMBL-AG | expression vector; contains BamHI site in eGFP stuffer |
| pETM-13/LIC | map | T7/lac | Kan | C-His | TEV | BL21 (DE3) | pBR322 | EMBL-AG | expression vector |
| pET GB-1a/LIC | map | T7/lac | Kan | N-His N-GB1 | TEV | BL21 (DE3) | pBR322 | EMBL-AG | expression vector |
| pET Z2-1a/LIC | map | T7/lac | Kan | N-His N-Z-tag2 | TEV | BL21 (DE3) | pBR322 | EMBL-AG | expression vector |
| pET TRXA-1a/LIC | map | T7/lac | Kan | N-His N-TrxA | TEV | BL21 (DE3) | pBR322 | EMBL-AG | expression vector |
| pET NusA-1a/LIC | map | T7/lac | Kan | N-His N-NusA | TEV | BL21 (DE3) | pBR322 | EMBL-AG | expression vector |
Source
EMBL-AG = EMBL-made vector by Arie Geerlof