STB -> PEPF -> Materials -> Vector database -> Creator vectors
Bacterial cloning and expression vectors for the Creator cloning technology
The pETC-K expression vectors are based on pET-24d(+)
| Vector | Vector map | Promoter | Antibiotic resistance | Tag(s) | Expression host | Origin of replication | Source | Description |
|---|---|---|---|---|---|---|---|---|
| pDNR-Dual | map | Amp | DH5α | pUC | Clontech | donor vector | ||
| pDNR-Dual/3C | map | Amp | DH5α | pUC | EMBL-AG | donor vector containing 3C protease cleavage site | ||
| pDNR-Dual/tev | map | Amp | DH5α | pUC | EMBL-AG | donor vector containing TEV protease cleavage site | ||
| pETC-10K | map | T7/lac | Kan | N-His | BL21 (DE3) | pBR322 | EMBL-AG | expression vector |
| pETC-20K | map | T7/lac | Kan | N-TrxA N-His | BL21 (DE3) | pBR322 | EMBL-AG | expression vector |
| pETC-30K | map | T7/lac | Kan | N-His N-GST | BL21 (DE3) | pBR322 | EMBL-AG | expression vector |
| pETC-40K | map | T7/lac | Kan | N-MBP | BL21 (DE3) | pBR322 | EMBL-AG | expression vector |
| pETC-41K | map | T7/lac | Kan | N-His N-MBP | BL21 (DE3) | pBR322 | EMBL-AG | expression vector |
| pETC-50K | map | T7/lac | Kan | N-DsbA N-His | BL21 (DE3) | pBR322 | EMBL-AG | expression vector |
| pETC-52K | map | T7/lac | Kan | N-leaderless DsbA N-His | BL21 (DE3) | pBR322 | EMBL-AG | expression vector |
| pETC-60K | map | T7/lac | Kan | N-NusA N-His | BL21 (DE3) | pBR322 | EMBL-AG | expression vector |
Source
EMBL-AG = EMBL-made vector by Arie Geerlof