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PEPP Facilities

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Equipment available at the Institute of Structural Biology (building 43b)

Innova 44R shaker/incubator

Bacterial cultivation

  • 3 New Brunswick Scientific Innova 44R shaker/incubator (Eppendorf)
     

Insect and mammalian cell cultivation

  • 2 New Brunswick Scientific Innova 42R shaker/incubator (Eppendorf)
  • HeraCell 150i incubator (Thermo Scientific)
  • New Brunswick Scientific S44i CO2-incubator (Eppendorf)
  • HeraSafe KS laminar flow hood (Thermo Scientific)
  • Microfluidics homogenizer

Cell lysis

  • Sonoplus HD2200 sonicator (Bandelin)
  • M110 homogenizer (Microfluidics)
     

Centrifugation

  • 2 Sorvall Lynx 6000 centrifuge (Thermo Scientific)
  • 2 5810R centrifuge (Eppendorf)
  • Äkta Purifier
     

Protein purification

  • 3 Äkta Purifier 10 (GE Healthcare)
  • 1 Äkta Pure (GE Healthcare)
     

Spectroscopy

  • Peqlab Nonodrop 2000 (Thermo Scientific)
  • 2 BioPhotometer plus (Eppendorf)
  • Viscotek TDA305 detector
     

Protein characterization

  • Viscotek TDA305 triple array detector (static light scattering) (Malvern Instruments)
  • MicroCal ITC200 MicroCalorimeter (GE Healthcare)
  • DynaPro Nanostar (dynamic light scattering) (Wyatt Technology)
  • Stratagene Mx3005P RT-PCR machine (Agilent Technologies)
     

Other equipment

  • C1000 ThermoCycler (Bio-Rad)
  • Savant SPD 111V SpeedVac concentrator (Thermo Scientific)
  • Micromodulyo Freeze Dryer (Thermo Scientific)