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EXSISERS_credit_Barth_van_Rossum
©Barth-Jan van Rossum

Proteins come in many flavors | high-throughput tracking of specific protein isoforms

ISBM,

We have generated a suite of gene reporters, EXSISERS, that self-excise themselves out of the native amino-acid chain of a protein of interest to report on the translation of specific protein isoforms from alternatively spliced mRNA.

 

 We have used EXSISERS for ratiometric monitoring of isoforms of the Tau protein. 

manuscript: https://doi.org/10.1038/s41556-021-00678-x

cover: https://www.nature.com/ncb/volumes/23/issues/6

press release: https://www.helmholtz-munich.de/en/newsroom/news-all/artikel/the-cut-and-restore-protein-trick-self-excising-designer-proteins-report-isoform-expression 

Please find a detailed protocol for EXSISERS here, 

https://protocolexchange.researchsquare.com/article/pex-1440/v1  

and the genetic constructs deposited here:

https://www.addgene.org/search/catalog/plasmids/?q=Westmeyer