Cloning and auxiliary vectors

PEPF Services Facilities Materials Protocols

STB -> PEPF -> Materials -> Vector database -> Bacterial cloning and auxiliary vectors

Bacterial cloning and auxiliary vectors

Vector	Vector map	Promoter	Antibiotic resistance	Tag(s)	Expression host	Origin of replication	Source	Description
pACYC 177	map		Amp Kan		DHα5	p15A	NEB	cloning vector
pET-24(+)	map	T7/lac	Kan	C-His	BL21 (DE3)	pBR322	Novagen	transcription vector; no ribosome binding site or transcription start codon
pJY1 pLysE-ArgU		T7 Tc	Cam		BL21 (DE3)	p15A	Affiniti	encodes rare ArgU codons encodes T7 lysosyme (higher concentration than in pJY2)
pJY2 pLysS-ArgU	map	T7 Tc	Cam		BL21 (DE3)	p15A	Affiniti	encodes rare ArgU codons encodes T7 lysosyme
pLysS	map	T5/lac	Cam		BL21	p15A	Qiagen	encodes T7 lysosyme that blocks leaky expression from T7 promoter by inhibiting T7 RNA polymerase
pPCR-Script	map	T7 T3	Amp		DHα5	ColE1	Stratagene	cloning vector
pPCR-Script eGFP	map	T7 T3	Amp		DHα5	ColE1	EMBL-AG	cloning vector; containing eGFP stuffer
pRARE	map		Cam			p15A	Novagen	encodes rare codon tRNAs: argU, argW, glyT, ileX, leuW, metT, proL, thrT, thrU and tyrU.
pRARE2	map		Cam			p15A	Novagen	encodes rare codon tRNAs: argU, argW, argX, glyT, ileX, leuW, metT, proL, thrT, thrU and tyrU.
pRARELysS			Cam			p15A	Novagen	encodes T7 lysosyme that blocks leaky expression from T7 promoter by inhibiting T7 RNA polymerase; encodes rare codon tRNAs: argU, argW, glyT, ileX, leuW, metT, proL, thrT, thrU and tyrU.
pREP4	map	unknown	Kan		M15	p15A	Qiagen	encodes lacI that blocks leaky expression before IPTG induction
pSP73	map	SP6	Amp		DHα5	unknown	Promega	cloning and in vitro transcription vector
pTA Plus	map	T7 T3	Amp		DHα5	ColE1	EMBL-AG	TA-cloning vector
pUBS ArgU amp			Amp			p15A		encodes rare ArgU codons
pUBS ArgU kan			Kan			p15A		encodes rare ArgU codons