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Bacterial cloning and auxiliary vectors

Vector Vector map Promoter Antibiotic resistance Tag(s) Expression host Origin of replication Source Description
pACYC 177 map   Amp
Kan		   DHα5 p15A NEB cloning vector
pET-24(+) map T7/lac Kan C-His BL21 (DE3) pBR322 Novagen transcription vector; no ribosome binding site or transcription start codon
pJY1
pLysE-ArgU		   T7
Tc		 Cam   BL21 (DE3) p15A Affiniti encodes rare ArgU codons
encodes T7 lysosyme (higher concentration than in pJY2)
pJY2
pLysS-ArgU		 map T7
Tc		 Cam   BL21 (DE3) p15A Affiniti encodes rare ArgU codons
encodes T7 lysosyme
pLysS map T5/lac Cam   BL21 p15A Qiagen encodes T7 lysosyme that blocks leaky expression from T7 promoter by inhibiting T7 RNA polymerase
pPCR-Script map T7
T3		 Amp   DHα5 ColE1 Stratagene cloning vector
pPCR-Script eGFP map T7
T3		 Amp   DHα5 ColE1 EMBL-AG cloning vector; containing eGFP stuffer
pRARE map   Cam     p15A Novagen encodes rare codon tRNAs: argU, argW, glyT, ileX, leuW, metT, proL, thrT, thrU and tyrU.
pRARE2 map   Cam     p15A Novagen encodes rare codon tRNAs: argU, argW, argX, glyT, ileX, leuW, metT, proL, thrT, thrU and tyrU.
pRARELysS     Cam     p15A Novagen encodes T7 lysosyme that blocks leaky expression from T7 promoter by inhibiting T7 RNA polymerase; encodes rare codon tRNAs: argU, argW, glyT, ileX, leuW, metT, proL, thrT, thrU and tyrU.
pREP4 map unknown Kan   M15 p15A Qiagen encodes lacI that blocks leaky expression before IPTG induction
pSP73 map SP6 Amp   DHα5 unknown Promega cloning and in vitro transcription vector
pTA Plus map T7
T3		 Amp   DHα5 ColE1 EMBL-AG TA-cloning vector
pUBS ArgU amp     Amp     p15A   encodes rare ArgU codons
pUBS ArgU kan     Kan     p15A   encodes rare ArgU codons