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STB -> PEPP -> Materials -> Vector database -> Gateway vectors

Gateway donor vectors

VectorVector mapPromoterAntibiotic resistanceTag(s)Expression hostOrigin of replicationSource
pDONR 201mapnoneKannone          DH5αpBR322Invitrogen
pDONR 207mapnoneGennoneDH5αpBR322Invitrogen
pDONR 221mapnone  DH5αpBR322Invitrogen
pENTR 4map   DH5αpBR322Invitrogen

 

Gateway expression vectors with Ampicillin resistance

The pETG-A expression vectors are based on pET-22b(+)

VectorVector mapPromoterAntibiotic resistanceTag(s)Expression hostOrigin of replicationSource
pDEST14mapT7Amp BL21 (DE3)pBR322Invitrogen
pDEST15mapT7AmpN-GSTBL21 (DE3)pBR322Invitrogen
pDEST17mapT7AmpN-HisBL21 (DE3)pBR322Invitrogen
pDEST17 I/OmapT7AmpN-HisBL21 (DE3)pBR322CNRS-MRS
pETG-10AmapT7/lacAmpN-HisBL21 (DE3)pBR322EMBL-AG
pETG-20AmapT7/lacAmpN-TrxA
N-His
BL21 (DE3)pBR322EMBL-AG
pETG-22AmapT7/lacAmpN-TrxA
N-Strep
BL21 (DE3)pBR322EMBL-AG
pETG-30AmapT7/lacAmpN-His
N-GST
BL21 (DE3)pBR322EMBL-AG
pETG-40AmapT7/lacAmpN-MBPBL21 (DE3)pBR322EMBL-AG
pETG-41AmapT7/lacAmpN-His
N-MBP
BL21 (DE3)pBR322EMBL-AG
pETG-50AmapT7/lacAmpN-DsbA
N-His
BL21 (DE3)pBR322EMBL-AG
pETG-52AmapT7/lacAmpN-leaderless-DsbA
N-His
BL21 (DE3)pBR322EMBL-AG
pETG-60AmapT7/lacAmpN-NusA
N-His
BL21 (DE3)pBR322EMBL-AG

pETG-80A

mapT7/lacAmpN-DsbC
N-His
BL21 (DE3)pBR322EMBL-AG

pETG-82A

mapT7/lacAmpN-leaderless-DsbC
N-His
BL21 (DE3)pBR322EMBL-AG

 

Gateway expression vectors with kanamycin resistance

The pETG-K expression vectors are based on pET-24d(+)

 

The pETG-K expression vectors are based on pET-24d(+)

VectorVector mapPromoterAntibiotic resistanceTag(s)Expression hostOrigin of replicationSource
pETG-10KmapT7/lacKanN-HisBL21 (DE3)pBR322EMBL-AG
pETG-20KmapT7/lacKanN-TrxA
N-His
BL21 (DE3)pBR322EMBL-AG
pETG-30KmapT7/lacKanN-His
N-GST
BL21 (DE3)pBR322EMBL-AG
pETG-40KmapT7/lacKanN-MBPBL21 (DE3)pBR322EMBL-AG
pETG-41KmapT7/lacKanN-His
N-MBP
BL21 (DE3)pBR322EMBL-AG
pETG-50KmapT7/lacKanN-DsbA
N-His
BL21 (DE3)pBR322EMBL-AG
pETG-52KmapT7/lacKanN-leaderless-DsbA
N-His
BL21 (DE3)pBR322EMBL-AG
pETG-60KmapT7/lacKanN-NusA
-His
BL21 (DE3)pBR322EMBL-AG

 

Source

EMBL-AG = EMBL-made vector by Arie Geerlof 
CNRS-MRS = vector made by Renaud Vincentelli at CNRS, Marseille, France