Sendai Reprogramming
Target of this service is the generation of iPSCs from PBMCs isolated from whole blood using Sendai virus.
Please email the completed submission form and provide a health certificate outlining that the health status of the donor is pathogen free (HIV-, HBV-, HCV-)
Sample submission: fresh whole blood/ freshly isolated PBMCs from whole blood.
Cryopreserved PBMCs (Note: the cryopreserved PBMCs will be processed for reprogramming unless the culture exhibits high percentage of cell death)
The service requires 4-6 months for completion.
Cell are transduced with Sendai virus and the reprogramming will be performed under feeder-free conditions.
Single-cell clones exhibiting the best morphologies and growth rates are further expanded for testing Sendai virus absence (TaqMan-based RT – PCR assay) and for cryopreservation.
Two Sendai negative clones are shipped to clients as frozen vials (5 vials per clone).
Sendai negative clones will be declared as BSL-1 (S1). The iPSCs are genetically modified organisms by definition despite the lack of foreign DNA, therefore customers require BSL-1/S1 laboratory for storing and handling the cells according to GenTSV.
Target of this service is the generation of iPSCs from PBMCs isolated from whole blood using Sendai virus.
Please email the completed submission form and provide a health certificate outlining that the health status of the donor is pathogen free (HIV-, HBV-, HCV-)
Sample submission: fresh whole blood/ freshly isolated PBMCs from whole blood.
Cryopreserved PBMCs (Note: the cryopreserved PBMCs will be processed for reprogramming unless the culture exhibits high percentage of cell death)
The service requires 4-6 months for completion.
Cell are transduced with Sendai virus and the reprogramming will be performed under feeder-free conditions.
Single-cell clones exhibiting the best morphologies and growth rates are further expanded for testing Sendai virus absence (TaqMan-based RT – PCR assay) and for cryopreservation.
Two Sendai negative clones are shipped to clients as frozen vials (5 vials per clone).
Sendai negative clones will be declared as BSL-1 (S1). The iPSCs are genetically modified organisms by definition despite the lack of foreign DNA, therefore customers require BSL-1/S1 laboratory for storing and handling the cells according to GenTSV.
