Targeted Lipidomics
Metabolomic ServiceLipidomic analyses cover fatty acid-derived hydrophobic metabolites that are not or only incompletely included in metabolomics panels. By extraction with unpolar solvents, a wide range of lipid species (Tri-/diacylglycerols, glycerophospholipids, sphingolipids, cholesterol esters etc.) become amenable to mass spectrometry-based targeted lipidomics methods. Visit the section "Shotgun Lipidomics", for more information about our targeted lipidomics assay.
We furthermore offer targeted lipidomic analyses of pro-inflammatory and pro-resolving lipid mediators (Prostaglandins, Leukotrienes, Lipoxins, Resolvins etc.) that are relevant in inflammatory processes. More information can be found under the section "Oxylipins".
We use the iLab Core Management system to book services by internal and external users.
Lipidomic analyses cover fatty acid-derived hydrophobic metabolites that are not or only incompletely included in metabolomics panels. By extraction with unpolar solvents, a wide range of lipid species (Tri-/diacylglycerols, glycerophospholipids, sphingolipids, cholesterol esters etc.) become amenable to mass spectrometry-based targeted lipidomics methods. Visit the section "Shotgun Lipidomics", for more information about our targeted lipidomics assay.
We furthermore offer targeted lipidomic analyses of pro-inflammatory and pro-resolving lipid mediators (Prostaglandins, Leukotrienes, Lipoxins, Resolvins etc.) that are relevant in inflammatory processes. More information can be found under the section "Oxylipins".
We use the iLab Core Management system to book services by internal and external users.
Our platforms
The DMS-SLA is a targeted Lipidomics platform that allows accurate quantification of up to 1508 (1582 including cardiolipins, CL) lipid species from 20 (21 with CL) lipid classes using differential mobility spectrometry (DMS) with selected reaction monitoring (SRM). Please note, that the actual number of lipid species that can be quantified is dependent on sample matrix and sample quality. In general, between 500 and 800 lipids are reliably quantified. The lipid quantification is based on known concentrations of internal standards (approx. 5 different fatty acids with various amount of carbon atoms and double-bonds per lipid class) that are spiked into the analytical samples prior the extraction procedure. The concentration values are reported as nmol/mL for liquids and nmol/g for tissues, respectively.
- Types of biological matrices: plasma, serum, cerebrospinal fluid (CSF), cell culture, brain, heart, kidney, lung, muscle, liver, pancreas, white adipose tissue (WAT), brown adipose tissue (BAT)
- Minimum amount of sample required: plasma/serum: 35 µl, preferably 50 µL, CSF: 200 µL. tissue: between 20-30 mg depending on the matrix
- Species tested: human and mouse
- Type of data provided: xlsx, containing lipid species concentrations in nmol/ml, nmol/g, or nmol/mg protein, xlsx file with derived values for lipid class concentrations, fatty acid concentrations, lipid species composition, lipid class composition, fatty acid composition, Rdata list-file containing all above mentioned values, HTML report
In the table below, biochemical classes and their number of lipids measured by the DMS-SLA are shown:
Biochemical classes and their number of lipids measured by the DMS-SLA | ||
Cholesterolester (26) | Dihydroceramide (12) | Ceramide (12) |
Diacylglycerol (81) | Free Fatty Acid (26) | Hexosylceramide (12) |
Lactosylceramide (12) | Lysophsphatidylcholine (26) | Lysophosphatidylethanolamine (26) |
Lysophosphatidylglycerol (26) | Lysophosphatidylglycerol (26) | Lysophosphatidylinositol (26) |
Lysophosphatidylserine (26) | Phosphatidic Acid (39) | Phosphatidylcholine (143) |
Phosphatidylethanolamine (216) | Phosphatidylglycerol (78) | Phosphatidylinositol (109) |
Phosphatidylserine (110) | Sphingomyelin (12) | Triacylglycerol (491) |
Cardiolipin (74; on request) |
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A complete list of the available lipids can be downloaded here.
The UHPLC-MS/MS method contains 58 oxylipins and PUFA precursors covering pro-inflammatory and pro-resolving lipid mediators from all known major pathways, i.e. COX- , LOX-and CYP450 mediated lipid conversion, as well as non-enzymatic oxidation. The identification and quantification are achieved by retention time and selected reaction monitoring (SRM), including 9-point calibration with 17 isotopically labelled internal standards.
- Types of biological matrices: plasma, serum, cell culture, brain, heart, kidney, lung, muscle, liver, white adipose tissue (WAT), brown adipose tissue (BAT)
- Minimum amount of sample required: for plasma/serum : 70 µl (better 100 µL); tissue: between 30-50 mg depending on the matrix
- Species tested: human and mouse
- Type of data supplied: xlsx, Rdata, txt-files containing lipid species concentrations in ng/ml, ng/mg
In the table below, biochemical classes and their number of lipids are shown:
Biochemical classes and their number of lipids measured by the Oxyplipin Assay | ||
DHET (3) | DiHOME (2) | EET (4) |
HDHA (4) | HEPE (4) | HETE (6) |
HETrE (1) | HODE (2) | Lipoxin (1) |
Maresin (1) | oxoETE (3) | pETE (3) |
Prostaglandin (5) | Protectin (1) | PUFA (6) |
Resolvin (4) |
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A complete list of the available lipids can be downloaded here.
