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Lipidomics

Metabolomic Service

Lipidomics analyses cover fatty acid-derived hydrophobic metabolites that are not or only incompletely included in metabolomics panels. By extraction with unpolar solvents, a wide range of lipid species (Tri-/diacylglyceroles, glycerophospholipids, sphingolipids, cholesterol esters etc.) become amenable to mass spectrometry-based targeted lipidomics methods. Visit the section "Lipidyzer", for more information about our Lipidomics assay.

We furthermore offer targeted lipidomics analyses of pro-inflammatory and pro-resolving lipid mediators (Prostaglandins, Leukotrienes, Lipoxins, Resolvins etc.) that are relevant in inflammatory processes. More information can be found under the section "Eicosanoids". 

We use the ilab Core Management system to book services by internal and external users. 

Lipidomics analyses cover fatty acid-derived hydrophobic metabolites that are not or only incompletely included in metabolomics panels. By extraction with unpolar solvents, a wide range of lipid species (Tri-/diacylglyceroles, glycerophospholipids, sphingolipids, cholesterol esters etc.) become amenable to mass spectrometry-based targeted lipidomics methods. Visit the section "Lipidyzer", for more information about our Lipidomics assay.

We furthermore offer targeted lipidomics analyses of pro-inflammatory and pro-resolving lipid mediators (Prostaglandins, Leukotrienes, Lipoxins, Resolvins etc.) that are relevant in inflammatory processes. More information can be found under the section "Eicosanoids". 

We use the ilab Core Management system to book services by internal and external users. 

Our platforms

The Lipidyzer/Shotgun Lipidomics is a targeted Lipidomics platform that allows accurate quantification of up to 1070 lipid species from 13 lipid classes using ion mobility mass spectrometry (IM-MS) with multiple reaction monitoring (MRM). Please note, that the actual number of lipid species that can be quantified is dependent on sample matrix and sample quality. In general, between 500 and 800 lipids are reliably quantified. The lipid quantification is based on known concentrations of internal standards (approx. 10 different fatty acids with various amount of carbon atoms and double-bonds per lipid class) that are spiked into the analytical samples prior the extraction procedure. The concentration values are reported as nmol/mL for liquids and nmol/g for tissues, respectively.

  • Types of samples measured: plasma, serum, cell culture, brain, heart, kidney, lung, muscle, liver, white adipose tissue, brown adipose tissue
  • Amount of sample requested for plasma/serum: 35 µl (better 50 µL); tissue: between 20-30 mg dependent on matrix
  • Species tested: human and mouse

A complete list of the available lipids can be downloaded here.

Lipid Class

Acronym

Number of Lipids

Cholesterolester

CE

26

Dihydroceramide

Cer d18:0

12

Ceramide

Cer d18:1

12

Diacylglycerol

DG

81

Free Fatty Acid

FFA

26

Hexosylceramide

HexCER

12

Lactosylceramide

LacCER

12

Lysophsphatidylcholine

LPC

26

Lysophosphatidylethanolamine

LPE

26

Lysophosphatidylglycerol

LPG

26

Lysophosphatidylinositol

LPI

25

Lysophosphatidylserine

LPS

26

Phosphatidic Acid

PA

39

Phosphatidylcholine

PC

143

Phosphatidylethanolamine

PE

180

Phosphatidylglycerol

PG

78

Phosphatidylinositol

PI

109

Phosphatidylserine

PS

110

Sphingomyelin

SM

12

 

The UHPLC-MS/MS method contains 58 oxylipins and PUFA precursors covering pro-inflammatory and pro-resolving lipid mediators from all known major pathways, i.e. COX- , LOX-, and CYP450 mediated lipid conversion, as well non-enzymatic oxidation.The identification and quantification is achieved by retention time and multiple reaction monitoring (MRM), including 9-point calibration with 17 isotopically labelled internal standards. 

  • Types of samples measured: plasma, serum, cell culture, brain, heart, kidney, lung, muscle, liver, white adipose tissue, brown adipose tissue
  • Minimum amount of sample requested for plasma/serum : 70 µl (better 100 µL); tissue: between 30-50 mg dependent on matrix
  • Species tested: human and mouse

A complete list of the available lipids can be downloaded here.

Eicosanoid Class

Number of lipids

Eicosanoid Class

Number of Lipids

DHET   

3

Lipoxin

1

DiHOME

2

Maresin

1

EET 

4

oxoETE

3

HDHA

4

pETE 

3

HEPE

4

Prostaglandin

5

HETE

6

Protectin

1

HETrE

1

PUFA   

6

HODE 

2

Resolvin

4

 

 

Our Contact

Membrane receptor in oncology cancer treatment, surface with the receptor within the bi lipid layer cell

Metabolomics Services Core Facility Metabolomics and Proteomics

Ingolstädter Landstraße 1 85764 Neuherberg, Building 25 and 34