Targeted Metabolomics
Metabolomic ServiceTargeted metabolomics describes the simultaneous measurement of a pre-selected set of metabolites. Therefore, assays are developed that comprise metabolites of one or more metabolic pathways of interest, i.e., there must be a priori knowledge about the object of investigation (e.g. a disease). Targeted metabolomics yields absolute quantification levels (concentrations) and can be used for biomarker discovery/validation and kinetic pathway analyses. We are using Biocrates® Assays and are the Certified Biocrates® Service Provider.
We use the iLab Core Management system to book services by internal and external users.
Targeted metabolomics describes the simultaneous measurement of a pre-selected set of metabolites. Therefore, assays are developed that comprise metabolites of one or more metabolic pathways of interest, i.e., there must be a priori knowledge about the object of investigation (e.g. a disease). Targeted metabolomics yields absolute quantification levels (concentrations) and can be used for biomarker discovery/validation and kinetic pathway analyses. We are using Biocrates® Assays and are the Certified Biocrates® Service Provider.
We use the iLab Core Management system to book services by internal and external users.
We offer the following assays for metabolite quantification:
This assay allows the determination of 630 metabolites from 26 biochemical classes using an assay kit from Biocrates® Life Sciences AG. Liquid handling is done on a Hamilton ML start liquid handling robot. The assay is based on LC-MS separation and detection (Agilent UHPLC, Sciex QTrap 5500) and flow injection analysis.
- Types of samples measured: plasma, serum, stool, cells
- Amount of sample requested: plasma: 50 µl minimum, stool: 200 mg
- Species tested: human and mouse
- Type of data supplied: csv/xlsx-files containing concentration data in µmol/l or µmol/g of the metabolites determined.
In the table below, biochemical classes and their number of metabolites measured applying the MxP® Quant 500 kit are shown:
Biochemical classes and their number of metabolites measured applying the MxP® Quant 500 kit | ||
Alkaloids (1) | Free fatty acids (12) | Ceramides (28) |
Amine oxides (1) | Hormones and related (4) | Dihydroceramides (8) |
Amino acids (20) | Indoles and derivatives (4) | Hexosylceramides (19) |
Amino acid related (30) | Nucleobases and related (2) | Dihexosylceramides (9) |
Bile acids (14) | Vitamins and cofactors (1) | Trihexosylceramides (6) |
Biogenic amines (9) | Acylcarnitines (40) | Cholesteryl esters (22) |
Carbohydrates and related (1) | Lysophosphatidylcholines (12) | Diglycerides (44) |
Carboxylic acids (7) | Phosphatidylcholines (78) | Triglycerides (242) |
Cresols (1) | Sphingomyelins (15) |
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Full list of metabolites covered by MxP® Quant 500 kit can be found here.
The assay quantifies different metabolites from 7 different metabolite classes using the Biocrates AbsoluteIDQTM technology. Liquid handling is done on a Hamilton ML Star liquid handling robot. Assays are based on LC-MS separation and detection (Agilent HPLC, CTC autosampler, and API 4000 MS/MS).
- Types of samples measured: plasma, serum, tissue, urine, cerebrospinal fluid (CSF), lung lavage
- Amount of sample requested: liquids: 50 µL minimum, preferably 100 µL, tissue: 25-50 mg minimum
- Species tested: human, mouse, rat, rabbit, hamster, lamb, bovine and zebrafish
Type of data supplied: csv/xlsx-files containing concentration data in µmol/l, pg/mg tissue, or µmol/g of the metabolites determined
In the table below, biochemical classes and their number of metabolites measured by the AbsoluteIDQ p180 Kit are shown:
Biochemical classes and their number of metabolites measured by the AbsoluteIDQ p180 Kit | ||
Free Carnitine (1) | Biogenic Amines (21) | Phosphatidylcholines (76) |
Acylcarnitines (39) | Hexose (sum of hexoses) (1) | Sphingomyelins (15) |
Amino Acids (21) | Lysophosphatidylcholines (14) |
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Full list of metabolites quantificate by Biocrates AbsoluteIDQ® p180 Kit can be found here.
Quantification of 20 bile acids is done using biocrates® AbsoluteIDQ® technology. Liquid handling is done on a Hamilton ML Star liquid handling robot. Assays are based on LC-MS/MS separation and detection (Agilent HPLC, CTC autosampler, and API 4000 MS/MS).
- Types of samples measured: plasma, serum, adipose tissue
- Minimal amount of sample requested: 50 µL
- Species tested: human, mouse, rat, hamster, sheep, bovine, porcine
- Type of data supplied: csv/xlsx-files containing concentration data in µmol/l, or pg/mg tissue or of the metabolites determined
Full list of bile acids quantificable by Biocrates AbsoluteIDQ® Kit can be found here.
