Targeted Metabolomics
Metabolomic ServiceThe term targeted metabolomics describes the simultaneous measurement of a pre-selected set of metabolites. Therefore, an assay is developed that comprises metabolites of one or more metabolic pathways of interest, i.e. there must be a priori knowledge about the object of investigation (e.g. a disease). Targeted metabolomics yields absolute quantification levels (concentrations) and can be used for biomarker discovery/validation and kinetic pathway analyses.
We use the ilab Core Management system to book services by internal and external users.
The term targeted metabolomics describes the simultaneous measurement of a pre-selected set of metabolites. Therefore, an assay is developed that comprises metabolites of one or more metabolic pathways of interest, i.e. there must be a priori knowledge about the object of investigation (e.g. a disease). Targeted metabolomics yields absolute quantification levels (concentrations) and can be used for biomarker discovery/validation and kinetic pathway analyses. We offer the following assays for metabolite quantification.
We use the ilab Core Management system to book services by internal and external users.
We offer the following assays for metabolite quantification:
This assay allows the determination of 630 metabolites from 26 biochemical classes using an assay kit from Biocrates® Life Sciences AG. Liquid handling is done on a ML Star Hamilton robot. The assay is based on LC-MS separation and detection (Agilent UHPLC, Sciex QTrap 5500). • Types of samples measured: plasma, serum, stool, cells • Amount of sample requested: plasma: 50 µl minimum, stool: 200 mg. • Species tested: human and mouse.
Metabolites determinable: download metabolite list MxP® Quant 500
The assay quantifies different metabolites from 7 different metabolite classes using the Biocrates AbsoluteIDQTM technology. Liquid handling is done on ML Star Hamilton robot. Assays are based on LC-MS separation and detection (Agilent HPLC, CTC autosampler and API 4000 MS/MS).
- Types of samples measured: plasma, serum, tissue, urine, cerebrospinal fluid (CSF), lung lavage.
- Amount of sample requested: liquids: 50 µL minimum, preferably 100 µL, tissue: 25-50 mg minimum.
- Species tested: human, mouse, rat, rabbit, hamster, lamb, bovine and zebrafish.
Kit p180 allows parallel quantification of more than 160 metabolites (Download Metabolite list p180)
Quantification of 20 bile acids is done using Biocrates AbsoluteIDQTM technology. Liquid handling is done on ML Star Hamilton robot. Assays are based on LC-MS/MS separation and detection (Agilent HPLC, CTC autosampler and API 4000 MS/MS).
Portfolio:
- Types of samples measured: plasma, serum, adipose tissue
- Minimal amount of sample requested: 50 µL
Bile Acids determinable: download metabolite list Bile Acid Kit
