©Barth-Jan van Rossum
Non-invasive monitoring of non-coding RNA | how to encode proteins from intronic information?
We introduce a new gene reporter system (INSPECT) that encode reporter or effector proteins from intronic information.
We introduce a new gene reporter system (INSPECT) that can dynamically monitor the expression of long non-coding RNAs (lncRNAs) without modifying the mature target RNA sequence. This capability is achieved by embedding INSPECT into an intron of the gene of interest, such that its post-transcriptional excision during RNA-splicing results in a synthetic transcript, which is exported out of the nucleus and translated into a reporter or effector protein of choice.
https://www.helmholtz-munich.de/en/newsroom/news-all/artikel/molecular-monitoring-of-rna-regulation
https://doi.org/10.1038/s41556-022-00998-6
