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©Barth-Jan van Rossum

Non-invasive monitoring of non-coding RNA | how to encode proteins from intronic information?


We introduce a new gene reporter system (INSPECT) that encode reporter or effector proteins from intronic information.

We introduce a new gene reporter system (INSPECT) that can dynamically monitor the expression of long non-coding RNAs (lncRNAs) without modifying the mature target RNA sequence. This capability is achieved by embedding INSPECT into an intron of the gene of interest, such that its post-transcriptional excision during RNA-splicing results in a synthetic transcript, which is exported out of the nucleus and translated into a reporter or effector protein of choice.