Dr. Ingo Burtscher
Stem Cell Research
The basis of in vitro differentiations are cellular and molecular processes occurring during early embryonic development. In our group we focus on a better understanding, how these processes lead to lineage specification, growth control and maturation of pancreatic endocrine lineages in the mouse. These findings we translate in vitro into the culture dish using induced pluripotent human stem cells (hiPSC).
First using mouse embryonic stem cells (mESCs) then using human induced pluripotent stem (iPSCs) cells Dr. Burtscher and his team generated numerous fluorescent reporter lines. These stem cell reporter lines are being used to understand how beta- or other pancreatic endocrine cell lineage form and how these lineage decisions can be influenced. Furthermore, they explore beta cell maturation which is closely related to their function. Applying various stress models, they try to understand how beta cell survival and glucose dependent insulin secretion are affected and how they can be restored.
Team Leader Beta Cell Replacement
Postdoc - Humboldt Fellow
Postdoc Stem Cell Research
PhD Student Stem Cell Research
PhD student - Beta cell replacement
PhD student - Intestinal Stem Cell Research
PhD Student - Beta cell development and regeneration
PhD Student Stem Cell Research
PhD student - Beta Cell Replacement
Lab Manager and TA Stem Cell Research
Publications
Greisle, T. ; Kunze, I. ; Wang, X. ; Malinowski, A.R. ; Böttcher, A. ; Lickert, H. ; Burtscher, I.
Generation of a Flattop-T2A-H2B-Venus x C-peptide-mCherry double reporter human iPSC line to monitor WNT/Planar cell polarity pathway activity.Singh, I. ; Onogi, Y. ; Menezes, F. ; Khasanova, D. ; Kang, L. ; Wang, C. ; Ruiz-Trave, J. ; Sharma, S. ; Khalil, A. ; Reichenbach, V.K. ; Shi, Y. ; Flatley, A. ; Yan, X. ; Israel, A. ; Dragano, N.R.V. ; Aguilar-Pimentel, J.A. ; Hoffmann, A. ; Ghosh, A. ; Noé, F. ; Wolfrum, C. ; Cucuruz, S. ; König, A.-C. ; Burtscher, I. ; Hauck, S.M. ; Lickert, H. ; Hofmann, S.M. ; Feederle, R. ; Schriever, S.C. ; Hernandez-Bautista, R. ; Sancar, G. ; Cebrian Serrano, A. ; Tetko, I. ; Fuchs, H. ; Gailus-Durner, V. ; Blüher, M. ; Hrabě de Angelis, M. ; Ussar, S.
NRAC controls CD36-mediated fatty acid uptake in adipocytes and lipid clearance in vivo.Setyono, E.S.A. ; Rogers, N.K. ; Hofmann, A. ; Lickert, H. ; Burtscher, I.
Generation of ARX-T2A-H2B-CFP x C-PEP-mCherry-hiPSC double reporter line for monitoring of pancreatic differentiation.Miranda-Cervantes, A. ; Fritzen, A.M. ; Raun, S.H. ; Hodek, O. ; Møller, L.L.V. ; Johann, K. ; Deisen, L. ; Gregorevic, P. ; Gudiksen, A. ; Artati, A. ; Adamski, J. ; Andersen, N.R. ; Sigvardsen, C.M. ; Carl, C.S. ; Voldstedlund, C.T. ; Kjøbsted, R. ; Hauck, S.M. ; Schjerling, P. ; Jensen, T.E. ; Cebrian Serrano, A. ; Jähnert, M. ; Gottmann, P. ; Burtscher, I. ; Lickert, H. ; Pilegaard, H. ; Schürmann, A. ; Tschöp, M.H. ; Moritz, T. ; Müller, T.D. ; Sylow, L. ; Kiens, B. ; Richter, E.A. ; Kleinert, M.
Pantothenate kinase 4 controls skeletal muscle substrate metabolism.Siehler, J. ; Bilekova, S. ; Chapouton, P. ; Dema, A. ; Albanese, P. ; Tamara, S. ; Jain, C. ; Sterr, M. ; Enos, S.J. ; Chen, C. ; Malhotra, C. ; Villalba, A. ; Schomann, L. ; Bhattacharya, S. ; Feng, J. ; Akgün, M. ; Ribaudo, F. ; Ansarullah ; Burtscher, I. ; Ahlbrecht, C. ; Plettenburg, O. ; Kurth, T. ; Scharfmann, R. ; Speier, S. ; Scheltema, R.A. ; Lickert, H.
Inceptor binds to and directs insulin towards lysosomal degradation in β cells.Hembach, S. ; Schmidt, S. ; Orschmann, T. ; Burtscher, I. ; Lickert, H. ; Giesert, F. ; Weisenhorn, D.M. ; Wurst, W.
Engrailed 1 deficiency induces changes in ciliogenesis during human neuronal differentiation.Rauch, E. ; Amendt, T. ; Lopez Krol, A. ; Lang, F.B. ; Linse, V. ; Hohmann, M. ; Keim, A.C. ; Kreutzer, S. ; Kawengian, K. ; Buchholz, M. ; Duschner, P. ; Grauer, S. ; Schnierle, B. ; Ruhl, A. ; Burtscher, I. ; Dehnert, S. ; Kuria, C. ; Kupke, A. ; Paul, S. ; Liehr, T. ; Lechner, M. ; Schnare, M. ; Kaufmann, A. ; Huber, M. ; Winkler, T.H. ; Bauer, S. ; Yu, P.
T-bet+ B cells are activated by and control endogenous retroviruses through TLR-dependent mechanisms.Cota, P. ; Caliskan, Ö.S. ; Bastidas-Ponce, A. ; Jing, C. ; Jaki, J. ; Saber, L. ; Czarnecki, O. ; Taskin, D. ; Blöchinger, A. ; Kurth, T. ; Sterr, M. ; Burtscher, I. ; Krahmer, N. ; Lickert, H. ; Bakhti, M.
Insulin regulates human pancreatic endocrine cell differentiation in vitro.Cota, P. ; Saber, L. ; Taskin, D. ; Jing, C. ; Bastidas-Ponce, A. ; Vanheusden, M. ; Shahryari, A. ; Sterr, M. ; Burtscher, I. ; Bakhti, M. ; Lickert, H.
NEUROD2 function is dispensable for human pancreatic β cell specification.Melzer, M.K. ; Schirge, S. ; Gout, J. ; Arnold, F. ; Srinivasan, D. ; Burtscher, I. ; Allgöwer, C. ; Mulaw, M. ; Zengerling, F. ; Günes, C. ; Lickert, H. ; Christoffels, V.M. ; Liebau, S. ; Wagner, M. ; Seufferlein, T. ; Bolenz, C. ; Moon, A.M. ; Perkhofer, L. ; Kleger, A.
TBX3 is dynamically expressed in pancreatic organogenesis and fine-tunes regeneration.Shahryari, A. ; Nazari, Z. ; Jazi, M.S. ; Hashemi-Shahraki, F. ; Wissmiller, K. ; Xu, W. ; Burtscher, I. ; Lickert, H.
Gene Therapy.Siehler, J. ; Chapouton, P. ; Bilekova, S. ; Ribaudo, F. ; Bhattacharia, S. ; Malhotra, C. ; Kurth, T. ; Burtscher, I. ; Lickert, H.
Inceptor binds and directs insulin to lysosomal degradation in beta cellsMoruzzi, N. ; Valladolid-Acebes, I. ; Kannabiran, S.A. ; Bulgaro, S. ; Burtscher, I. ; Leibiger, B. ; Leibiger, I.B. ; Berggren, P.O. ; Brismar, K.
Mitochondrial impairment and intracellular reactive oxygen species alter primary cilia morphology.Bakhti, M. ; Bastidas-Ponce, A. ; Tritschler, S. ; Czarnecki, O. ; Tarquis Medina, M. ; Nedvedova, E. ; Jaki, J. ; Willmann, S. ; Scheibner, K. ; Cota, P. ; Salinno, C. ; Boldt, K. ; Horn, N. ; Ueffing, M. ; Burtscher, I. ; Theis, F.J. ; Coskun, Ü. ; Lickert, H.
Synaptotagmin-13 orchestrates pancreatic endocrine cell egression and islet morphogenesis.Shahryari, A. ; Moya, N. ; Siehler, J. ; Wang, X. ; Burtscher, I. ; Lickert, H.
Increasing gene editing efficiency for CRISPR-Cas9 by small RNAs in pluripotent stem cells.Scheibner, K. ; Schirge, S. ; Burtscher, I. ; Büttner, M. ; Sterr, M. ; Yang, D. ; Böttcher, A. ; Ansarullah ; Irmler, M. ; Beckers, J. ; Cernilogar, F.M. ; Schotta, G. ; Theis, F.J. ; Lickert, H.
Publisher Correction: Epithelial cell plasticity drives endoderm formation during gastrulation.Shahryari, A. ; Burtscher, I. ; Nazari, Z. ; Lickert, H.
Engineering gene therapy: Advances and barriers.Scheibner, K. ; Schirge, S. ; Burtscher, I. ; Büttner, M. ; Sterr, M. ; Yang, D. ; Böttcher, A. ; Ansarullah ; Irmler, M. ; Beckers, J. ; Cernilogar, F.M. ; Schotta, G. ; Theis, F.J. ; Lickert, H.
Epithelial cell plasticity drives endoderm formation during gastrulation.Böttcher, A. ; Büttner, M. ; Tritschler, S. ; Sterr, M. ; Aliluev, A. ; Oppenländer, L. ; Burtscher, I. ; Sass, S. ; Irmler, M. ; Beckers, J. ; Ziegenhain, C. ; Enard, W. ; Schamberger, A.C. ; Verhamme, F.M. ; Eickelberg, O. ; Theis, F.J. ; Lickert, H.
Author Correction: Non-canonical Wnt/PCP signalling regulates intestinal stem cell lineage priming towards enteroendocrine and Paneth cell fates.Suwandhi, L. ; Altun, I. ; Karlina, R. ; Miok, V. ; Wiedemann, T. ; Fischer, D.S. ; Walzthoeni, T. ; Lindner, C. ; Böttcher, A. ; Heinzmann, S.S. ; Israel, A. ; Khalil, A. ; Braun, A. ; Pramme-Steinwachs, I. ; Burtscher, I. ; Schmitt-Kopplin, P. ; Heinig, M. ; Elsner, M. ; Lickert, H. ; Theis, F.J. ; Ussar, S.
Asc-1 regulates white versus beige adipocyte fate in a subcutaneous stromal cell population.Contact