Single Cell Genomics
Dr. Michael SterrSingle-cell based methods are key technologies to uncover the cellular complexity of biological systems, answering important and longstanding biological questions. Single-cell genomics allows to better characterize different cell types and cellular states, identify rare cell populations, and reconstruct developmental trajectories.
Single-cell based methods are key technologies to uncover the cellular complexity of biological systems, answering important and longstanding biological questions. Single-cell genomics allows to better characterize different cell types and cellular states, identify rare cell populations, and reconstruct developmental trajectories.
Research Topics
The enteroendocrine cells found in the intestinal epithelium secrete a multitude of hormones that are essential for the regulation of energy and glucose homeostasis and food intake. However, the mechanistic details, regulating their differentiation from intestinal stem cells remain elusive. Using multimodal single-cell genomics, we dissect the regulatory mechanisms of endocrine cell fate formation in the intestine.
Pancreatic beta cells secrete insulin upon food intake to regulate blood glucose levels. In diabetes, beta cell function is lost, which ultimately leads to a loss of beta cell mass. To restore functional beta cell mass, two promising approaches that are currently investigated are the regeneration from existing tissue or the replacement with stem-cell derived beta cells. The foundation of both strategies is a detailed understanding of pancreas and endocrine cell formation during embryonic development. To achieve this, we study mouse and porcine pancreas development using single-cell technologies.
Stem cell-derived pancreatic islets are a promising source of insulin producing beta cells for replacement therapy of diabetes. To produce functionally mature beta cells, it is important to understand in-vivo endocrine formation and apply developmental mechanisms in-vitro. At the same time, the results of the differentiation protocols have to be analyzed in great detail to identify potential improvement measures. The analysis of single-cell genomics data is an important step in this process.
The enteroendocrine cells found in the intestinal epithelium secrete a multitude of hormones that are essential for the regulation of energy and glucose homeostasis and food intake. However, the mechanistic details, regulating their differentiation from intestinal stem cells remain elusive. Using multimodal single-cell genomics, we dissect the regulatory mechanisms of endocrine cell fate formation in the intestine.
Pancreatic beta cells secrete insulin upon food intake to regulate blood glucose levels. In diabetes, beta cell function is lost, which ultimately leads to a loss of beta cell mass. To restore functional beta cell mass, two promising approaches that are currently investigated are the regeneration from existing tissue or the replacement with stem-cell derived beta cells. The foundation of both strategies is a detailed understanding of pancreas and endocrine cell formation during embryonic development. To achieve this, we study mouse and porcine pancreas development using single-cell technologies.
Stem cell-derived pancreatic islets are a promising source of insulin producing beta cells for replacement therapy of diabetes. To produce functionally mature beta cells, it is important to understand in-vivo endocrine formation and apply developmental mechanisms in-vitro. At the same time, the results of the differentiation protocols have to be analyzed in great detail to identify potential improvement measures. The analysis of single-cell genomics data is an important step in this process.
Who we are
Publications
Read more2022 Scientific Article in Diabetes
Heterogeneous development of β-cell populations In diabetes-resistant and -susceptible mice.
2022 Scientific Article in Glia
Diet triggers specific responses of hypothalamic astrocytes in time and region dependent manner.
2022 Scientific Article in Clinical Cancer Research
Therapy-related transcriptional subtypes in matched primary and recurrent head and neck cancer.
2021 Scientific Article in International Journal of Molecular Sciences
Synaptotagmin-13 is a neuroendocrine marker in brain, intestine and pancreas.
2021 Scientific Article in Molecular Metabolism
Vertical sleeve gastrectomy triggers fast β-cell recovery upon overt diabetes.
2021 Scientific Article in Nature metabolism
Diet-induced alteration of intestinal stem cell function underlies obesity and prediabetes in mice.
2021 Nature Cell Biology
Publisher Correction: Epithelial cell plasticity drives endoderm formation during gastrulation.
2021 Scientific Article in Nature biomedical engineering
Single-cell-resolved differentiation of human induced pluripotent stem cells into pancreatic duct-like organoids on a microwell chip.
2021 Scientific Article in Nature Cell Biology
Epithelial cell plasticity drives endoderm formation during gastrulation.
2021 Nature Cell Biology
Author Correction: Non-canonical Wnt/PCP signalling regulates intestinal stem cell lineage priming towards enteroendocrine and Paneth cell fates.
2021 Nature
Author Correction: Inceptor counteracts insulin signalling in β-cells to control glycaemia.
