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Helmholtz Munich | Winfried Meining

Sattler Lab

We are using nuclear magnetic resonance (NMR) spectroscopy in  integrative structural biology, combined with X-ray crystallography, SAXS, SANS, cryo-EM and biophysical techniques to study the structure, interactions and dynamics of biomolecules in solution and for structure-based drug discovery.

We are using nuclear magnetic resonance (NMR) spectroscopy in  integrative structural biology, combined with X-ray crystallography, SAXS, SANS, cryo-EM and biophysical techniques to study the structure, interactions and dynamics of biomolecules in solution and for structure-based drug discovery.

About

Molecular recognition in the regulation of gene expression and signaling

A main focus in the Sattler group is to understand the structural basis of protein-RNA interactions that are functionally important for various aspects of gene expression, such as the regulation of (alternative) pre-mRNA splicing and gene silencing by non-coding RNAs (siRNAs, miRNAs). More than 90% of human multi-exon genes are alternatively spliced and misregulation of splicing is linked to various human diseases. The spliceosome is a highly dynamic machinery, which involves numerous protein-RNA interactions. During the different steps that eventually lead to splicing of the pre-mRNA, these complexes are continuously rearranged, and their composition can be modulated, for example in the context of alternative splicing. While this requires that the molecular interactions are dynamic, specific and tight complexes are formed by the cooperative combination of multiple weak protein-protein and protein-RNA interactions. Current projects focus on protein-protein and protein-RNA interactions that play important roles in the regulation of constitutive and alternative splicing and other aspects of RNA metabolism involving non-coding RNAs.

NMR is well suited to study such dynamic and transient interactions in solution. We are implementing and developing integrated structural biology approaches combining NMR-spectroscopy and Small Angle Neutron and/or X-ray Scattering (SAXS/SANS) data with crystallographic and other information to investigate molecular mechanisms involving high molecular weight protein complexes in solution.

Another area of research is structural investigations of proteins and protein complexes involved in different aspects of cellular signaling and peroxisomal biogenesis. Here, we aim to understand the structural basis for critical protein-protein interactions with a focus on proteins that are linked to human disease.

We are also initiating studies for the structure-based design of small molecular inhibitors as i) starting points for pharmaceutical interference and ii) as tools to modulate and monitor cellular signaling. These studies aim at identifying optimized small chemical compounds using structure-guided approaches. NMR is an efficient tool for such a structure-based chemical biology approach since it not only allows to determine the three-dimensional structures in solution but also is efficient in detecting and mapping ligand binding of biomolecules.

Group members

Prof. Dr. Michael Sattler

Head of the Molecular Targets and Therapeutics Centers, Director Structural Biology View profile

Karen Biniossek

Assistant to Prof. Dr. Michael Sattler

Dr. Sam Asami

Staff scientist

Dr. Stefan Bohn

Staff scientist

Dr. Matthias Brandl

Staff scientist

PD Dr. Gerd Gemmecker

Staff scientist

Dr. Winfried Meining

IT manager

Gülden Yilmaz

Lab manager

Dr. Peijian Zou

Staff scientist

Dr. Lorenzo Baronti

Postdoc

Dr. Jan Borggräfe

Postdoc

Dr. Florent Delhommel

Postdoc

Dr. Tony Fröhlich

Postdoc

Dr. Stefan Gaussmann

Postdoc

Dr. Hyunseo Kang

Postdoc

Dr. Santiago Martinez-Lumbreras

Postdoc

Dr. Patrick O'Connor

Postdoc

Annika Elimelech

PhD student

Philipp Mayer

PhD student

Clara Hipp

PhD student

Nitin Kachariya

PhD student

Niki Messini

PhD student

Christoph Müller-Hermes

PhD student

Luca Sperotto

PhD student

Ekaterina Tikhaia

PhD student

Clara Morguet

PhD student

Sebastian Metzler

Master student

Stefanie Seuß

Master student

Fadime Stemmer

Bachelor's student

Jessica Walther

Technical assistant

Publications

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Sattler Lab Contact

Karen Biniossek

Assistant to Prof. Dr. Michael Sattler

TUM Garching, BNMRZ