Sattler Lab
We are using nuclear magnetic resonance (NMR) spectroscopy in integrative structural biology, combined with X-ray crystallography, SAXS, SANS, cryo-EM and biophysical techniques to study the structure, interactions and dynamics of biomolecules in solution and for structure-based drug discovery.
We are using nuclear magnetic resonance (NMR) spectroscopy in integrative structural biology, combined with X-ray crystallography, SAXS, SANS, cryo-EM and biophysical techniques to study the structure, interactions and dynamics of biomolecules in solution and for structure-based drug discovery.
About
Molecular recognition in the regulation of gene expression and signaling
A main focus in the Sattler group is to understand the structural basis of protein-RNA interactions that are functionally important for various aspects of gene expression, such as the regulation of (alternative) pre-mRNA splicing and gene silencing by non-coding RNAs (siRNAs, miRNAs). More than 90% of human multi-exon genes are alternatively spliced and misregulation of splicing is linked to various human diseases. The spliceosome is a highly dynamic machinery, which involves numerous protein-RNA interactions. During the different steps that eventually lead to splicing of the pre-mRNA, these complexes are continuously rearranged, and their composition can be modulated, for example in the context of alternative splicing. While this requires that the molecular interactions are dynamic, specific and tight complexes are formed by the cooperative combination of multiple weak protein-protein and protein-RNA interactions. Current projects focus on protein-protein and protein-RNA interactions that play important roles in the regulation of constitutive and alternative splicing and other aspects of RNA metabolism involving non-coding RNAs.
NMR is well suited to study such dynamic and transient interactions in solution. We are implementing and developing integrated structural biology approaches combining NMR-spectroscopy and Small Angle Neutron and/or X-ray Scattering (SAXS/SANS) data with crystallographic and other information to investigate molecular mechanisms involving high molecular weight protein complexes in solution.
Another area of research is structural investigations of proteins and protein complexes involved in different aspects of cellular signaling and peroxisomal biogenesis. Here, we aim to understand the structural basis for critical protein-protein interactions with a focus on proteins that are linked to human disease.
We are also initiating studies for the structure-based design of small molecular inhibitors as i) starting points for pharmaceutical interference and ii) as tools to modulate and monitor cellular signaling. These studies aim at identifying optimized small chemical compounds using structure-guided approaches. NMR is an efficient tool for such a structure-based chemical biology approach since it not only allows to determine the three-dimensional structures in solution but also is efficient in detecting and mapping ligand binding of biomolecules.
Group members
Head of the Molecular Targets and Therapeutics Centers, Director Structural Biology, Molecular Targets and Therapeutics Center, Institute of Structural Biology
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Assistant to Prof. Dr. Michael Sattler
Staff Scientist
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IT manager
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Technical assistant
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PhD student
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Publications
Lorenz, S. ; Wahida, A. ; Bostock, M.J. ; Seibt, T. ; Santos Dias Mourão, A. ; Levkina, A. ; Trümbach, D. ; Soudy, M. ; Emler, D. ; Rothammer, N. ; Woo, M.S. ; Sonner, J.K. ; Novikova, M. ; Henkelmann, B. ; Aldrovandi, M. ; Kaemena, D.F. ; Mishima, E. ; Vermonden, P. ; Zong, Z. ; Cheng, D. ; Nakamura, T. ; Ito, J. ; Doll, S. ; Proneth, B. ; Bürkle, E. ; Rizzollo, F. ; Escamilla Ayala, A. ; Napolitano, V. ; Kolonko, M. ; Gaussmann, S. ; Merl-Pham, J. ; Hauck, S.M. ; Pertek, A. ; Orschmann, T. ; Van San, E. ; Vanden Berghe, T. ; Hass, D, ; Maida, A. ; Frenz, J.M. ; Pedrera, L. ; Dolga, A.M. ; Kraiger, M. ; Hrabě de Angelis, M. ; Fuchs, H. ; Ebert, G. ; Lenberg, J. ; Friedman, J. ; Scale, C. ; Agostinis, P. ; Zimprich, A. ; Vogt Weisenhorn, D.M. ; Garrett, L. ; Hölter, S.M. ; Wurst, W. ; Glaab, E. ; Lewerenz, J. ; Popper, B. ; Sieben, C. ; Steinacker, P. ; Zischka, H. ; García-Sáez, A.J. ; Tietze, A. ; Ramesh, S.K. ; Ayton, S. ; Vincendeau, M. ; Friese, M.A. ; Wigby, K. ; Sattler, M. ; Mann, M. ; Ingold, I. ; Jayavelu, A.K. ; Popowicz, G.M. ; Conrad, M.
A fin-loop-like structure in GPX4 underlies neuroprotection from ferroptosis.Wagner Egea, P. ; Delhommel, F. ; Mustafa, G. ; Leiss-Maier, F. ; Klimper, L. ; Badmann, T. ; Heider, A. ; Wille, I. ; Groll, M. ; Sattler, M. ; Zeymer, C.
Modular protein scaffold architecture and AI-guided sequence optimization facilitate de novo metalloenzyme engineering.Bostock, M.J. ; Kolloff, C. ; Jerschke, E. ; Asami, S. ; Skerra, A. ; Olsson, S. ; Sattler, M.
Conformational quenching in an engineered lipocalin protein achieves high affinity binding to the toxin colchicine.Pérez-Ràfols, A. ; Pérez-Ropero, G. ; Cerofolini, L. ; Sperotto, L. ; Roca-Martínez, J. ; Higuera-Rodriguez, R.A. ; Russomanno, P. ; Kaiser, W.J. ; Vranken, W.F. ; Danielson, U.H. ; Provenzani, A. ; Martelli, T. ; Sattler, M. ; Buijs, J. ; Fragai, M.
Deciphering the RNA recognition by Musashi-1 to design protein and RNA variants for in vitro and in vivo applications.Hagl, B. ; Spielberger, B.D. ; Neumann, B. ; Pelham, S.J. ; Pandey, D. ; Schlundt, A. ; Barro, C. ; Lechner, A. ; Wolf, C. ; Deenick, E.K. ; Sattler, M. ; Tangye, S.G. ; Rothenfusser, S. ; Renner, E.D.
Signal transducer and activator of transcription 3 (STAT3) variant p.K709N causes hyper-IgE syndrome likely by impaired STAT3-dimer formation.Jobs
Research Practicals, "Mitarbeit am Arbeitsplatz"
Practicals and lab rotations are available in protein / RNA biochemistry, (basic) NMR experiments and analysis of protein or RNA spectra.
Please send a notice (including a short CV) to Hanso Kang (hyunseo.kang (a) tum.de) for further information.
Bachelor or Master theses
Bachelor and master thesis projects are available in the area of biological NMR, integrative structural biology (NMR, crystallography, SAXS/SANS, cryo-EM) and biochemical studies of protein complexes, RNA and protein-RNA as well as protein small molecule interactions. Please directly contact Michael Sattler (michael.sattler@tum.de)
Doctoral theses and postdoc positions
We are always looking for excellent, highly motivated doctoral students and postdoctoral fellows.
Doctoral thesis and postdoctoral research projects are available in different aspects of our research:
- Biochemistry and structural biology of proteins, RNA linked to RNA biology (splicing, non-coding RNAs
- Biochemistry and structural biology of Hsp90/complexes, peroxisome biogenesis and cellular signalling
- NMR methods, integrated structural biology (i.e. combining NMR, crystallography, cryo-EM, SAXS/SANS)
- Structure-based drug discovery
Please directly contact Michael Sattler (michael.sattler@tum.de) for inquiries.
Current job openings
- Doctoral thesis "Biochemistry, structural analysis and drug discovery of lncRNAs in cancer"
- Doctoral thesis "Temperature-dependent phase separation of RNA binding proteins in chloroplasts" at HU Berlin (joint with TUM)
in the SPP219 "Molecular Mechanisms of Functional Phase Separation" joint project TUM/HU Berlin project - Doctoral thesis "Structural and functional investigation of the protein C19orf12 involved in the neurodegenerative disease MPAN"
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