Aerosol Mutagenesis investigates the genotoxic and mutagenic potential of aerosols and nanomaterials that potentially affect human health.

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About our research

The research group Aerosol Mutagenesis aims to detect, characterize and depict the mechanism of action of physical, chemical, and biological aerosol components in inducing genetic and epigenetic changes in vitro related to environmental and occupational inhalation exposures.

New requirements in the (epi) genotoxicity testing of different aerosol phases are therefore taken into account both with regard to the adaptation of cytogenetic assays to ALI exposures (Air-Liquid Interface) and with regard to cell-based model improvements for assessing health-related risks.

The overarching aim is to Replace, Reduce and Refine (3R) animal testing with improved airborne (nano) particle and fiber exposure systems, which retain the contribution of the aerosol gas phase to the induction of mutagenic effects. This challenging approach allows us to consider the role of atmospheric oxidation of aerosol´s volatile organic compounds in differentiating secondary organic aerosol cytogenetic effects with respect to primary emissions. Additionally, advanced co-culture systems, which allow a variety of cell types to be cultured together in the ALI, will enhance the understanding of secondary (epi) genotoxic mechanisms of action and thus implying genetic damage caused by oxidative burst during an aerosol-elicited inflammation.

Overall, these insights contribute to an increased knowledge about the complexity of environmental factors effects on human health.

ULTRHAS - ULtrafine particles from TRansportation – Health Assessment of Sources

The EU-funded ULTRHAS project aims to disclose the health threats posed by Ultrafine Particles (UFPs) emitted from different transport modes, and provide guidance for policy development to improve air quality and health.

aeroHEALTH

In aeroHEALTH the toxicity of polluted air is analyzed. A major step into real world scenarios is made by taking into consideration the interactions of anthropogenic and biogenic emissions (e.g. car emissions and α-pinene from trees) and atmospheric aging of aerosols.

Toxicity of SVOC gas- and particle phase

Semi-volatile organic compounds (SVOCs) can be inhaled in gas or particle phase. By using aerosol generators and air-liquid-interface cell exposure systems it is investigated whether gas-particle partitioning influences the (geno-)toxicity of SCOCs.

Assessment of oxidative stress in cells exposed to fine particulate matter

Oxidative stress in cell systems caused by different exposure scenarios is used to study and elucidate molecular effect mechanisms.

Biogenic Organotropic Wetsuits (BOW)

Extracellular vesicles are transport all-rounders whose properties shall be exploited for superparamagnetic nanodevices. In this way, the researchers aim to advance the sustainable production and clinical transferability of implantable nanodevices and materials.

Dr. Sebastiano Di Bucchianico

Head of Aerosol Mutagenesis

Mohammad Almasaleekh

PhD Student

Evelyn Kuhn

Chemical Engineer

Svenja Offer

PhD Student

Jana Pantzke

PhD Student

Publications

2022 Scientific Article in Toxics Toxics 10:730 (2022)

Binder, S. ; Rastak, N. ; Karg, E.W. ; Huber, A. ; Kuhn, E. ; Dragan, G.C. ; Monsé, C. ; Breuer, D. ; Di Bucchianico, S. ; Delaval, M.N. ; Oeder, S. ; Sklorz, M. ; Zimmermann, R.

Construction of an in vitro air–liquid interface exposure system to assess the toxicological impact of gas and particle phase of semi-volatile organic compounds.

2022 Scientific Article in Toxicology Mechanisms and Methods Toxicol. Mech. Methods, DOI: 10.1080/15376516.2022.2156008 (2022)

Pantzke, J. ; Offer, S. ; Zimmermann, E. ; Kuhn, E. ; Streibel, T. ; Oeder, S. ; Di Bucchianico, S. ; Zimmermann, R.

An alternative in vitro model considering cell-cell interactions in fiber-induced pulmonary fibrosis.

2022 Scientific Article in Fibers Fibers 10:39 (2022)

Koch, A. ; Friederici, L. ; Fiala, P. ; Springer, A. ; Di Bucchianico, S. ; Stintz, M. ; Frank, M. ; Rüger, C.P. ; Streibel, T. ; Zimmermann, R.

Impact of thermal stress on abrasive dust from a carbon fiber-reinforced concrete composite.

2022 Scientific Article in Environment International Environ. Int. 166:107366 (2022)

Pardo, M. ; Offer, S. ; Hartner, E. ; Di Bucchianico, S. ; Bisig, B. ; Bauer, S. ; Pantzke, J. ; Zimmermann, E. ; Cao, X. ; Binder, S. ; Kuhn, E. ; Huber, A. ; Jeong, S. ; Käfer, U. ; Schneider, E. ; Mesceriakovas, A. ; Bendl, J. ; Brejcha, R. ; Buchholz, A. ; Gat, D. ; Hohaus, T. ; Rastak, N. ; Karg, E.W. ; Jakobi, G. ; Kalberer, M. ; Kanashova, T. ; Hu, Y. ; Ogris, C. ; Marsico, A. ; Theis, F.J. ; Shalit, T. ; Gröger, T.M. ; Rüger, C.P. ; Oeder, S. ; Orasche, J. ; Paul, A. ; Ziehm, T. ; Zhang, Z.H. ; Adam, T. ; Sippula, O. ; Sklorz, M. ; Schnelle-Kreis, J. ; Czech, H. ; Kiendler-Scharr, A. ; Zimmermann, R. ; Rudich, Y.

Exposure to naphthalene and β-pinene-derived secondary organic aerosol induced divergent changes in transcript levels of BEAS-2B cells.

2022 Scientific Article in Orphanet Journal of Rare Diseases : OJRD Orphanet J. Rare Dis. 17:179 (2022)

Rus, C.M. ; Weissensteiner, T. ; Pereira, C. ; Susnea, I. ; Danquah, B.D. ; Morales Torres, G. ; Rocha, M.E. ; Cozma, C. ; Saravanakumar, D. ; Mannepalli, S. ; Kandaswamy, K.K. ; Di Bucchianico, S. ; Zimmermann, R. ; Rolfs, A. ; Bauer, P. ; Beetz, C.

Clinical and genetic characterization of a cohort of 97 CLN6 patients tested at a single center.

2022 Scientific Article in Mutation Research / Genetic Toxicology and Environmental Mutagenesis Mutat. Res. - Gen. Tox. Environ. Mutag. 874-875:503446 (2022)

Cao, X. ; Padoan, S. ; Binder, S. ; Bauer, S. ; Orasche, J. ; Rus, C.M. ; Mudan, A. ; Huber, A. ; Kuhn, E. ; Oeder, S. ; Lintelmann, J. ; Adam, T. ; Di Bucchianico, S. ; Zimmermann, R.

A comparative study of persistent DNA oxidation and chromosomal instability induced in vitro by oxidizers and reference airborne particles.

2022 Scientific Article in Environmental Health Perspectives Environ. Health Perspect. 130:27003 (2022)

Offer, S. ; Hartner, E. ; Di Bucchianico, S. ; Bisig, B. ; Bauer, S. ; Pantzke, J. ; Zimmermann, E. ; Cao, X. ; Binder, S. ; Kuhn, E. ; Huber, A. ; Jeong, S. ; Käfer, U. ; Martens, P. ; Mesceriakovas, A. ; Bendl, J. ; Brejcha, R. ; Buchholz, A. ; Gat, D. ; Hohaus, T. ; Rastak, N. ; Jakobi, G. ; Kalberer, M. ; Kanashova, T. ; Hu, Y. ; Ogris, C. ; Marsico, A. ; Theis, F.J. ; Pardo, M. ; Gröger, T.M. ; Oeder, S. ; Orasche, J. ; Paul, A. ; Ziehm, T. ; Zhang, Z.H. ; Adam, T. ; Sippula, O. ; Sklorz, M. ; Schnelle-Kreis, J. ; Czech, H. ; Kiendler-Scharr, A. ; Rudich, Y. ; Zimmermann, R.

Effect of atmospheric aging on soot particle toxicity in lung cell models at the air-liquid interface: Differential toxicological impacts of biogenic and anthropogenic Secondary Organic Aerosols (SOAs).

2022 Scientific Article in Science of the Total Environment, The Sci. Total Environ. 806:150489 (2022)

Ihantola, T. ; Hirvonen, M.R. ; Ihalainen, M. ; Hakkarainen, H. ; Sippula, O. ; Tissari, J. ; Bauer, S. ; Di Bucchianico, S. ; Rastak, N. ; Hartikainen, A. ; Leskinen, J. ; Yli-Pirilä, P. ; Martikainen, M.V. ; Miettinen, M. ; Suhonen, H. ; Rönkkö, T.J. ; Kortelainen, M. ; Lamberg, H. ; Czech, H. ; Martens, P. ; Orasche, J. ; Michalke, B. ; Yildirim, A.Ö. ; Jokiniemi, J. ; Zimmermann, R. ; Jalava, P.I.

Genotoxic and inflammatory effects of spruce and brown coal briquettes combustion aerosols on lung cells at the air-liquid interface.

2021 Scientific Article in Exposure and Health Expo. Health, DOI: 10.1007/s12403-021-00429-1 (2021)

Gawlitta, N. ; Zimmermann, E. ; Orasche, J. ; Huber, A. ; Buters, J.T.M. ; Di Bucchianico, S. ; Oeder, S. ; Gröger, T.M. ; Zimmermann, R.

Impact of volatile and semi-volatile organic compounds from farming environments on allergy-related cellular processes.

2021 Scientific Article in Environmental and Molecular Mutagenesis Environ. Mol. Mutagen., DOI: 10.1002/em.22464 (2021)

Binder, S. ; Cao, X. ; Bauer, S. ; Rastak, N. ; Kuhn, E. ; Dragan, G.C. ; Monsé, C. ; Ferron, G.A. ; Breuer, D. ; Oeder, S. ; Karg, E.W. ; Sklorz, M. ; Di Bucchianico, S. ; Zimmermann, R.

In vitro genotoxicity of dibutyl phthalate on A549 lung cells at air-liquid interface in exposure concentrations relevant at workplaces.

2021 Scientific Article in Metabolites Metabolites 11:382 (2021)

Rus, C.M. ; Di Bucchianico, S. ; Cozma, C. ; Zimmermann, R. ; Bauer, P.

Dried Blood Spot (Dbs) methodology study for biomarker discovery in Dried Blood Spot (lsd).

2021 Scientific Article in Aerosol Science and Technology Aerosol Sci. Technol. 5, 292–306 (2021)

Koch, A. ; Bergelt, P. ; Fiala, P. ; Käfer, U. ; Orasche, J. ; Bauer, S. ; Di Bucchianico, S. ; Stintz, M. ; Gröger, T.M. ; Streibel, T. ; Zimmermann, R.

Investigation of chemical composition and fiber-occurrence in inhalable particulate matter obtained from dry cutting processes of carbon fiber reinforced concrete composite, concrete and the carbon fiber reinforcement materials.

2021 Scientific Article in Analytical Biochemistry Anal. Biochem. 618:114127 (2021)

Cao, X. ; Lintelmann, J. ; Padoan, S. ; Bauer, S. ; Huber, A. ; Mudan, A. ; Oeder, S. ; Adam, T. ; Di Bucchianico, S. ; Zimmermann, R.

Adenine derivatization for LC-MS/MS epigenetic DNA modifications studies on monocytic THP-1 cells exposed to reference particulate matter.

2020 Scientific Article in Particle and Fibre Toxicology Part. Fibre Toxicol. 17:27 (2020)

Ihantola, T. ; Di Bucchianico, S. ; Happo, M. ; Uski, O. ; Bauer, S. ; Kuuspalo, K. ; Sippula, O. ; Tissari, J. ; Oeder, S. ; Hartikainen, A. ; Rönkkö, T.J. ; Martikainen, M.-V. ; Huttunen, K. ; Vertiainen, P. ; Suhonen, H. ; Kortelainen, M. ; Lamberg, H. ; Leskinen, A. ; Sklorz, M. ; Michalke, B. ; Dilger, M. ; Weiss, C. ; Dittmar, G. ; Beckers, J. ; Irmler, M. ; Buters, J.T.M. ; Cadeias, J. ; Czech, H. ; Yli-Pirilä, P. ; Abbaszade, G. ; Jakobi, G. ; Orasche, J. ; Schnelle-Kreis, J. ; Kanashova, T. ; Karg, E.W. ; Streibel, T. ; Passig, G. ; Hakkarainen, H. ; Jokiniemi, J. ; Zimmermann, R. ; Hirvonen, M.-R. ; Jalava, P.I.

Influence of wood species on toxicity of log-wood stove combustion aerosols: A parallel animal and air-liquid interface cell exposure study on spruce and pine smoke.

2020 Scientific Article in Nanomaterials Nanomaterials 10:649 (2020)

Gliga, A.R. ; Di Bucchianico, S. ; Akerlund, E. ; Karlsson, H.L.

Transcriptome profiling and toxicity following long-term, low dose exposure of human lung cells to Ni and NiO nanoparticles – a comparison to NiCl2.

2020 Scientific Article in Nanomaterials Nanomaterials 10:618 (2020)

Cappellini, F. ; Di Bucchianico, S. ; Karri, V. ; Latvala, S. ; Malmlöf, M. ; Kippler, M. ; Elihn, K. ; Hedberg, J. ; Wallinder, I.O. ; Gerde, P. ; Karlsson, H.L.

Dry generation of CeO2 nanoparticles and deposition onto a co-culture of A549 and THP-1 cells in air-liquid interface – dosimetry consideration and comparison to submerged exposure.

2020 Scientific Article in Aerosol Science and Technology Aerosol Sci. Technol. 54, 668-684 (2020)

Karg, E.W. ; Ferron, G.A. ; Bauer, S. ; Di Bucchianico, S. ; Zimmermann, R.

Aerosol Mutagenesis

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About our research

Specific Topics

ULTRHAS - ULtrafine particles from TRansportation – Health Assessment of Sources

aeroHEALTH

Toxicity of SVOC gas- and particle phase

Assessment of oxidative stress in cells exposed to fine particulate matter

Biogenic Organotropic Wetsuits (BOW)

Scientists at Aerosol Mutagenesis

Dr. Sebastiano Di Bucchianico

Mohammad Almasaleekh

Evelyn Kuhn

Svenja Offer

Jana Pantzke

Publications

Construction of an in vitro air–liquid interface exposure system to assess the toxicological impact of gas and particle phase of semi-volatile organic compounds.

An alternative in vitro model considering cell-cell interactions in fiber-induced pulmonary fibrosis.

Impact of thermal stress on abrasive dust from a carbon fiber-reinforced concrete composite.

Exposure to naphthalene and β-pinene-derived secondary organic aerosol induced divergent changes in transcript levels of BEAS-2B cells.

Clinical and genetic characterization of a cohort of 97 CLN6 patients tested at a single center.

A comparative study of persistent DNA oxidation and chromosomal instability induced in vitro by oxidizers and reference airborne particles.

Effect of atmospheric aging on soot particle toxicity in lung cell models at the air-liquid interface: Differential toxicological impacts of biogenic and anthropogenic Secondary Organic Aerosols (SOAs).

Genotoxic and inflammatory effects of spruce and brown coal briquettes combustion aerosols on lung cells at the air-liquid interface.

Impact of volatile and semi-volatile organic compounds from farming environments on allergy-related cellular processes.

In vitro genotoxicity of dibutyl phthalate on A549 lung cells at air-liquid interface in exposure concentrations relevant at workplaces.

Dried Blood Spot (Dbs) methodology study for biomarker discovery in Dried Blood Spot (lsd).

Investigation of chemical composition and fiber-occurrence in inhalable particulate matter obtained from dry cutting processes of carbon fiber reinforced concrete composite, concrete and the carbon fiber reinforcement materials.

Adenine derivatization for LC-MS/MS epigenetic DNA modifications studies on monocytic THP-1 cells exposed to reference particulate matter.

Influence of wood species on toxicity of log-wood stove combustion aerosols: A parallel animal and air-liquid interface cell exposure study on spruce and pine smoke.

Transcriptome profiling and toxicity following long-term, low dose exposure of human lung cells to Ni and NiO nanoparticles – a comparison to NiCl2.

Dry generation of CeO2 nanoparticles and deposition onto a co-culture of A549 and THP-1 cells in air-liquid interface – dosimetry consideration and comparison to submerged exposure.

Is the particle deposition in a cell exposure facility comparable to the lungs? A computer model approach.

Determination of DNA damage and modifications in cells exposed to particulate matter.

Charakterisierung von Partikelemissionen aus dem Trennschleifprozess von kohlefaserverstärktem Beton (Carbonbeton).

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Dr. Sebastiano Di Bucchianico

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