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Aerosol Mutagenesis

Aerosol Mutagenesis investigates the genotoxic and mutagenic potential of aerosols and nanomaterials that potentially affect human health.

Aerosol Mutagenesis investigates the genotoxic and mutagenic potential of aerosols and nanomaterials that potentially affect human health.

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About our research

The research group Aerosol Mutagenesis aims to detect, characterize and depict the mechanism of action of physical, chemical, and biological aerosol components in inducing genetic and epigenetic changes in vitro related to environmental and occupational inhalation exposures.

New requirements in the (epi) genotoxicity testing of different aerosol phases are therefore taken into account both with regard to the adaptation of cytogenetic assays to ALI exposures (Air-Liquid Interface) and with regard to cell-based model improvements for assessing health-related risks.

The overarching aim is to Replace, Reduce and Refine (3R) animal testing with improved airborne (nano) particle and fiber exposure systems, which retain the contribution of the aerosol gas phase to the induction of mutagenic effects. This challenging approach allows us to consider the role of atmospheric oxidation of aerosol´s volatile organic compounds in differentiating secondary organic aerosol cytogenetic effects with respect to primary emissions. Additionally, advanced co-culture systems, which allow a variety of cell types to be cultured together in the ALI, will enhance the understanding of secondary (epi) genotoxic mechanisms of action and thus implying genetic damage caused by oxidative burst during an aerosol-elicited inflammation. 

Overall, these insights contribute to an increased knowledge about the complexity of environmental factors effects on human health.

Scientists at Aerosol Mutagenesis

Dr. Sebastiano Di Bucchianico

Head of Aerosol Mutagenesis

Mohammad Almasaleekh

PhD Student

Evelyn Kuhn

Chemical Engineer

Svenja Offer

PhD Student

Jana Pantzke

PhD Student

Publications

2022 Scientific Article in Environment International Environ. Int. 166:107366 (2022)

Pardo, M. ; Offer, S. ; Hartner, E. ; Di Bucchianico, S. ; Bisig, B. ; Bauer, S. ; Pantzke, J. ; Zimmermann, E. ; Cao, X. ; Binder, S. ; Kuhn, E. ; Huber, A. ; Jeong, S. ; Käfer, U. ; Schneider, E. ; Mesceriakovas, A. ; Bendl, J. ; Brejcha, R. ; Buchholz, A. ; Gat, D. ; Hohaus, T. ; Rastak, N. ; Karg, E.W. ; Jakobi, G. ; Kalberer, M. ; Kanashova, T. ; Hu, Y. ; Ogris, C. ; Marsico, A. ; Theis, F.J. ; Shalit, T. ; Gröger, T.M. ; Rüger, C.P. ; Oeder, S. ; Orasche, J. ; Paul, A. ; Ziehm, T. ; Zhang, Z.H. ; Adam, T. ; Sippula, O. ; Sklorz, M. ; Schnelle-Kreis, J. ; Czech, H. ; Kiendler-Scharr, A. ; Zimmermann, R. ; Rudich, Y.

Exposure to naphthalene and β-pinene-derived secondary organic aerosol induced divergent changes in transcript levels of BEAS-2B cells.

2022 Scientific Article in Mutation Research / Genetic Toxicology and Environmental Mutagenesis Mutat. Res. - Gen. Tox. Environ. Mutag. 874-875:503446 (2022)

Cao, X. ; Padoan, S. ; Binder, S. ; Bauer, S. ; Orasche, J. ; Rus, C.M. ; Mudan, A. ; Huber, A. ; Kuhn, E. ; Oeder, S. ; Lintelmann, J. ; Adam, T. ; Di Bucchianico, S. ; Zimmermann, R.

A comparative study of persistent DNA oxidation and chromosomal instability induced in vitro by oxidizers and reference airborne particles.

2022 Scientific Article in Environmental Health Perspectives Environ. Health Perspect. 130:27003 (2022)

Offer, S. ; Hartner, E. ; Di Bucchianico, S. ; Bisig, B. ; Bauer, S. ; Pantzke, J. ; Zimmermann, E. ; Cao, X. ; Binder, S. ; Kuhn, E. ; Huber, A. ; Jeong, S. ; Käfer, U. ; Martens, P. ; Mesceriakovas, A. ; Bendl, J. ; Brejcha, R. ; Buchholz, A. ; Gat, D. ; Hohaus, T. ; Rastak, N. ; Jakobi, G. ; Kalberer, M. ; Kanashova, T. ; Hu, Y. ; Ogris, C. ; Marsico, A. ; Theis, F.J. ; Pardo, M. ; Gröger, T.M. ; Oeder, S. ; Orasche, J. ; Paul, A. ; Ziehm, T. ; Zhang, Z.H. ; Adam, T. ; Sippula, O. ; Sklorz, M. ; Schnelle-Kreis, J. ; Czech, H. ; Kiendler-Scharr, A. ; Rudich, Y. ; Zimmermann, R.

Effect of atmospheric aging on soot particle toxicity in lung cell models at the air-liquid interface: Differential toxicological impacts of biogenic and anthropogenic Secondary Organic Aerosols (SOAs).

2022 Scientific Article in Science of the Total Environment, The Sci. Total Environ. 806:150489 (2022)

Ihantola, T. ; Hirvonen, M.R. ; Ihalainen, M. ; Hakkarainen, H. ; Sippula, O. ; Tissari, J. ; Bauer, S. ; Di Bucchianico, S. ; Rastak, N. ; Hartikainen, A. ; Leskinen, J. ; Yli-Pirilä, P. ; Martikainen, M.V. ; Miettinen, M. ; Suhonen, H. ; Rönkkö, T.J. ; Kortelainen, M. ; Lamberg, H. ; Czech, H. ; Martens, P. ; Orasche, J. ; Michalke, B. ; Yildirim, A.Ö. ; Jokiniemi, J. ; Zimmermann, R. ; Jalava, P.I.

Genotoxic and inflammatory effects of spruce and brown coal briquettes combustion aerosols on lung cells at the air-liquid interface.

2021 Scientific Article in Aerosol Science and Technology Aerosol Sci. Technol. 5, 292–306 (2021)

Koch, A. ; Bergelt, P. ; Fiala, P. ; Käfer, U. ; Orasche, J. ; Bauer, S. ; Di Bucchianico, S. ; Stintz, M. ; Gröger, T.M. ; Streibel, T. ; Zimmermann, R.

Investigation of chemical composition and fiber-occurrence in inhalable particulate matter obtained from dry cutting processes of carbon fiber reinforced concrete composite, concrete and the carbon fiber reinforcement materials.

2020 Scientific Article in Particle and Fibre Toxicology Part. Fibre Toxicol. 17:27 (2020)

Ihantola, T. ; Di Bucchianico, S. ; Happo, M. ; Uski, O. ; Bauer, S. ; Kuuspalo, K. ; Sippula, O. ; Tissari, J. ; Oeder, S. ; Hartikainen, A. ; Rönkkö, T.J. ; Martikainen, M.-V. ; Huttunen, K. ; Vertiainen, P. ; Suhonen, H. ; Kortelainen, M. ; Lamberg, H. ; Leskinen, A. ; Sklorz, M. ; Michalke, B. ; Dilger, M. ; Weiss, C. ; Dittmar, G. ; Beckers, J. ; Irmler, M. ; Buters, J.T.M. ; Cadeias, J. ; Czech, H. ; Yli-Pirilä, P. ; Abbaszade, G. ; Jakobi, G. ; Orasche, J. ; Schnelle-Kreis, J. ; Kanashova, T. ; Karg, E.W. ; Streibel, T. ; Passig, G. ; Hakkarainen, H. ; Jokiniemi, J. ; Zimmermann, R. ; Hirvonen, M.-R. ; Jalava, P.I.

Influence of wood species on toxicity of log-wood stove combustion aerosols: A parallel animal and air-liquid interface cell exposure study on spruce and pine smoke.

Head of Aerosol Mutagenesis

Contact

Dr. Sebastiano Di Bucchianico

Head of Aerosol Mutagenesis

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