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CRISPR/Cas9 iPSC Editing

Target of the service is the generation of knock-out (KO) pluripotent stem cell lines using Cas9-gRNAribonucleoproteins (RNPs) complex.   

Please email the completed submission form and provide information outlining that the pluripotent stem cell lines are mycoplasma-negative and pathogen-free (HIV-, HBV-, HCV-).

Sample submission: cryopreserved pluripotent stem cell lines.

The service requires 2-4 months for completion.

The cells will be nucleofected with RNPs complex targeting the locus of interest and 2-4 KO single cell clones will be generated for expansion and cryopreservation.

Remark: Feel free to contact us if you wish to use alternative Crispr/Cas9 applications from the one described above.

Target of the service is the generation of knock-out (KO) pluripotent stem cell lines using Cas9-gRNAribonucleoproteins (RNPs) complex.   

Please email the completed submission form and provide information outlining that the pluripotent stem cell lines are mycoplasma-negative and pathogen-free (HIV-, HBV-, HCV-).

Sample submission: cryopreserved pluripotent stem cell lines.

The service requires 2-4 months for completion.

The cells will be nucleofected with RNPs complex targeting the locus of interest and 2-4 KO single cell clones will be generated for expansion and cryopreservation.

Remark: Feel free to contact us if you wish to use alternative Crispr/Cas9 applications from the one described above. 

Our contacts

Core Facility Induced Pluripotent Stem Cells

Ingolstädter Landstraße 1 85764 Neuherberg, Building 35.34, Room 217