CRISPR/Cas9 iPSC Editing
Target of the service is the generation of knock-out (KO) pluripotent stem cell lines using Cas9-gRNAribonucleoproteins (RNPs) complex.
Please email the completed submission form and provide information outlining that the pluripotent stem cell lines are mycoplasma-negative and pathogen-free (HIV-, HBV-, HCV-).
Sample submission: cryopreserved pluripotent stem cell lines.
The service requires 2-4 months for completion.
The cells will be nucleofected with RNPs complex targeting the locus of interest and 2-4 KO single cell clones will be generated for expansion and cryopreservation.
Remark: Feel free to contact us if you wish to use alternative Crispr/Cas9 applications from the one described above.
Target of the service is the generation of knock-out (KO) pluripotent stem cell lines using Cas9-gRNAribonucleoproteins (RNPs) complex.
Please email the completed submission form and provide information outlining that the pluripotent stem cell lines are mycoplasma-negative and pathogen-free (HIV-, HBV-, HCV-).
Sample submission: cryopreserved pluripotent stem cell lines.
The service requires 2-4 months for completion.
The cells will be nucleofected with RNPs complex targeting the locus of interest and 2-4 KO single cell clones will be generated for expansion and cryopreservation.
Remark: Feel free to contact us if you wish to use alternative Crispr/Cas9 applications from the one described above.