Microscopy Services

The Zeiss 880 upright laser scanning confocal microscope can be used for multicolour fluorescence imaging as well as for live-cell imaging. The system is equipped with 2 conventional photomultiplier tubes and a high sensitive GaAsP detector as well as an airyscan detector for higher resolution confocal scanning (resolution in x,y,z increased by a factor of max. 1.7) and a FAST-Scan Modul.

Microscope:

• Zeiss Axio Examiner.Z1

Ilumination:

• HXP 120V (visual observation)
• LSM880 laser modules:
  • Diode laser 405 nm
  • Argon Multiline laser 458 / 488 / 514 nm
  • Laser 561 nm
  • HeNe laser 594 / 633 nm

Objectives:

• 10x/0.45NA Plan-Apochromat (a=2.0 mm)
• 20x/0.8NA Plan-Apochromat (a=0.55 mm)
• 20x/1.0NA W Plan-Apochromat DIC (a=1.7 mm)
• 63x/1.4NA Oil Plan-Apochromat DIC (UV)VIS-IR (a=0.13 mm)
• 63x/1.2NA W Korr C-Apochromat (a=0.28 mm)

Detectors:

• 2 Photomultiplier tubes
• 1 GaAsP Detector
• 1 Airyscan detector

Software:

• ZEN Black 2.3

Fluorescence filter sets:

For visual observation filtersets for DAPI, GFP, Cy 3 and Cy 5 are available.

The Celldiscoverer 7 is highly automated boxed inverted LSM900 with Airyscan 2, dedicated to high content screening projects a well as imaging under consistent conditions.  You can use a widefield or confocal mode. The guided acquisition module enables fully automated imaging setups with initial large widefield overviews, image analysis to identify obcects of interest in the overviews and subsequent high-resolution imaging, z-stacks of those identified positions.

Microscope:

• automated inverted confocal microscope (LSM900 with Airyscan 2)
• widefield with LED fluorescence and transmitted light
• slide holders for slides, dishes and plates
• incubation / fully integrated climate control
• automated stage and focus

Light sources:

• Colibri 7 LED (385nm/420nm/470nm/520/567nm/590nm/625nm)
• LSM900 Diodenlaser (405nm, 488nm, 561nm, 640nm)

Objectives:

• 5x/0.35 Plan-Apochromat (WD 3.99 mm)
• 20x/0.7 autocorr Plan-Apochromat (WD 1.33 mm)
• 20x/0.95 autocorr Plan-Apochromat (WD 0.4 mm)
• 50x/1.2W autocorr Plan-Apochromat (WD 0.4mm)

Afocal magnification changer (0.5x / 1.0x / 2.0x) is available for all objectives

The 50x is equipped with automated water dispenser.

Camera System:

• AxioCam 512 mono,  64 bit, pixel size 3.1 µm, 4250 x 239 pixel

Software:

• ZEN Blue 3.7

Fluorescence filters:

The Zeiss AxioImager.Z1 light microscope offers acquisition of conventional fluorescence images as well as optical sectioning with an Apotome system.

Microscope:

• Zeiss AxioImager.Z1

Ilumination:

• X-CITE 120 Mercury short arc lamp
• XBO 75 Xenon short arc lamp

Objectives:

• 2.5x/0.075 Plan-Neofluar
• 10x/0.3 Plan-Neofluar
• 20x/0.5 Plan-Neofluar
• 40x/1.2 W C-Apochromat
• 63x/1.2 W C-Apochromat

Camera:

• Zeiss AxioCam MRm

Software:

• Zeiss AxioVision 4.8

Fluorescence filter sets:

This 3D lightsheet fluorescence microscope (LSFM) - also called Single / Selective Plane Imaging Microscopes (SPIM) - provides high speed optical sectioning of large cleared samples, such as whole mouse brains, at a cellulare magnification.

This system is equipped with a white light laser which provides a broad range of excitation wavelengths, potentially allowing us to excite fluorophores with excitation wavelengths even in infrared. This microscope is optimized for the imaging of large cleared samples.

Microscope:

• LaVision UltraMicroscope II, Blaze (Miltenyi BioTec)

Ilumination:

• Dual side illumination with triple sheet optics on each side generating laser light sheet perpendicular to the direction of detection (Minimum thickness: <4.5 μm @ 488 nm for one individual sheet).
• SuperK EXTREME EXW12 White light laser system with a wavelength range from 465 to 2400 nm.

Fluorescence filter sets:

Optics:

Objektive: advanced light sheet objectives lenses covering thr redactive index range from 1.33 to 1.56.

Software controlled, motorized optical post-magnification changer (0.6x, 1x, 1.66x, 2.5x). Total magnification range from 0.66x up to 30x.

Camera:

Andor Zyla 4.2 Plus sCMOS camera (2048 x 2048 Pixel, 6.5 x 6.5 µm² Pixel size

Software:

LaVision BioTec ImSpector Software

The Axio Scan 7 is a powerful slidescanner for high throughput automated image acquisition of brightfield as well as multi-channel fluorescence slides up to NIR.

Additionally it's equipped with a phase and relief contrast module (TIE - Transfer of Intensity Equation) which helps to detect transparent tissue or protect samples with sensitive fluorescent dyes from bleaching.

Axio Scan 7 features high numerical aperture dry objectives for high resolution and is able to scan whole 3D volumes.

Microscope:

• automated upright slide scanning microscope
• slide holders for standard slides (26 x 76mm) or big slides (52 x 76mm)

Ilumination:

• transmitted light LED (Colibri 7: 385 nm / 430 nm / 475 nm / 555 nm / 590 nm / 630 nm / 735 nm)

Objectives:

• 5x/0.25NA Plan-Fluar (a=12.5 mm) M27
• 10x/0.45NA Plan-Apochromat (a=2.1 mm) M27
• 20x/0.5NA EC Plan-Neofluar M27 (a=2 mm) (not suitable for fluorescence)
• 20x/0.8NA Plan-Apochromat (a=0.55 mm) M27
• 40x/0.95NA Plan-Apochromat (a=0.25 mm) M27

Fluorescence filter sets:

*Can be used only alternatively, not in combination

Camera:

• AxioCam 712 mono, 14 bit, pixel size 3.45 µm, 4096 x 3008 Pixel
• AxioCam 705 color, 14 bit color camera, pixel size 3.45 µm, 2464 x 2056 pixel

Software:

• ZEN Blue 3.5

• Visiopharm, AI based software tool for standardized, quantitative image analysis of histological section
• ZEN Blue for Image Postprocessing and Analysis
• Visison 4D (ARIVIS) for xD visualization and quantitative structural analysis of tissue morphology