Cell Engineering
Stiel Lab
Imaging is our window into the processes of life – we bioengineer genetically encoded protein-based tools for optical imaging such as optoacoustics and super resolution microscopy.
Stiel Lab
Imaging is our window into the processes of life – we bioengineer genetically encoded protein-based tools for optical imaging such as optoacoustics and super resolution microscopy.
About our research group
The Cell Engineering group is devoted to the development of novel reporters and sensors for Optoacoustic (OA) imaging and other innovative imaging methods like super-resolution fluorescence microscopy. Our main focus is on genetically encoded contrast agents since only such agents allow longitudinal and unperturbed imaging in research animal to truly understand biology and diseases contexts.
To develop novel genetically encoded tools we use molecular engineering concepts that incorporate knowledge from photo-physical studies, protein structures and computational protein design and combine those insights with efficient screening concepts to identify novel reporters or sensors for the next pressing questions in whole animal imaging.
Technological developments
Besides our strong research in protein engineering, we also engage in the development of infrastructure for optoacoustic spectroscopy and screening. For example, we developed ...
Fields of Work and Expertise
Label and Sensor EngineeringFunctional Understanding (Structural and Photophysically)Highthroughput Screening Techniques for Directed Evolution
Our Researchers
Publications
Englert, L. ; Perleberg, B. ; Tayal, S. ; Wang, B. ; Huang, Y. ; Azeem, M. ; He, H. ; Stiel, A.-C. ; Ntziachristos, V.
Assessment of performance characteristics of photoswitchable bacteriophytochromes at different depths using optoacoustic mesoscopy.Menchicchi, B. ; Stiel, A.-C. ; Nieddu, M. ; Fuenzalida-Werner, J.P.
Fluorescent proteins: A journey from the cell to extreme environments in material science.Aguirre Bueno, J. ; Gasteiger, C. ; Hindelang, B. ; Seeger, M. ; Berezhnoi, A. ; Weidenfeld, I. ; Darsow, U. ; Stiel, A.-C. ; Steimle-Grauer, S.A. ; Posch, C. ; Biedermann, T. ; Ntziachristos, V.
Non-invasive characterization of melanoma depth at single-cell resolution.Huang, Y. ; Stankevych, M. ; Gujrati, V. ; Klemm, U. ; Mohammed, A. ; Wiesner, D. ; Saccomano, M. ; Tost, M. ; Feuchtinger, A. ; Mishra, K. ; Bruns, O.T. ; Geerlof, A. ; Ntziachristos, V. ; Stiel, A.-C.
Photoswitching protein-XTEN fusions as injectable optoacoustic probes.Göllner, S. ; Colditz, M. ; Huang, Y. ; Schmidt, H. ; Winkler, A.S. ; Stiel, A.-C.
Chip-based optoacoustic single cell detection in flow using point-source optimized surface acoustic wave transducers.Liu, Y. ; Chuah, J. ; Huang, Y. ; Stiel, A.-C. ; Unser, M. ; Dong, J.
Model-based temporal unmixing towards quantitative photo-switching optoacoustic tomography.Stiel, A.-C. ; Ntziachristos, V.
Controlling the sound of light: photoswitching optoacoustic imaging.Huang, S. ; He, H. ; Tom, R.Z. ; Glasl, S. ; Anzenhofer,P. ; Stiel, A.-C. ; Hofmann, S.M. ; Ntziachristos, V.
Non-invasive optoacoustic imaging of dermal microcirculatory revascularization in diet-induced obese mice undergoing exercise intervention.Hasler, M. ; Patrian, M. ; Banda-Vázquez, J.A. ; Ferrara, S. ; Stiel, A.-C. ; Fuenzalida-Werner, J.P. ; Costa, R.D.
Fully biogenic near-infrared phosphors: Phycobiliproteins and cellulose at force toward highly efficient and stable bio-hybrid light-emitting diodes.Liu, N. ; Gujrati, V. ; Fuenzalida Werner, J.P. ; Mishra, K. ; Anzenhofer,P. ; Stiel, A.-C. ; Mettenleiter, G. ; Feuchtinger, A. ; Walch, A.K. ; Ntziachristos, V.
Bacterial outer membrane vesicles as cationic dye carriers for optoacoustics-guided phototherapy of cancer.Rodrigues, E.M. ; Stiel, A.-C.
It's a two-way street: Photoswitching and reversible changes of the protein matrix in photoswitchable fluorescent proteins and bacteriophytochromes.Göllner, S. ; Mishra, K. ; Stiel, A.-C.
Schallschalter: Photoschaltbare Reporter und Sensoren in der Optoakustik.Liu, N. ; Mishra, K. ; Stiel, A.-C. ; Gujrati, V. ; Ntziachristos, V.
The sound of drug delivery: Optoacoustic imaging in pharmacology.Fuenzalida Werner, J.P. ; Mishra, K. ; Stankevych, M. ; Klemm, U. ; Ntziachristos, V. ; Stiel, A.-C.
Alginate beads as a highly versatile test-sample for optoacoustic imaging.Yun, M. ; You, S.H. ; Nguyen, V.H. ; Prakash, J. ; Glasl, S. ; Gujrati, V. ; Choy, H.E. ; Stiel, A.-C. ; Min, J.J. ; Ntziachristos, V.
Reporter gene-based optoacoustic imaging of E. coli targeted colon cancer in vivo.Mishra, K. ; Fuenzalida Werner, J.P. ; Pennacchietti, F. ; Janowski, R. ; Chmyrov, A. ; Huang, Y. ; Zakian Dominguez, C.M. ; Klemm, U. ; Testa, I. ; Niessing, D. ; Ntziachristos, V. ; Stiel, A.-C.
Genetically encoded photo-switchable molecular sensors for optoacoustic and super-resolution imaging.Stankevych, M. ; Mishra, K. ; Ntziachristos, V. ; Stiel, A.-C.
A practical guide to photoswitching optoacoustics tomography.Liu, N. ; Gujrati, V. ; Malekzadeh-Najafabadi, J. ; Werner, J.P,F. ; Klemm, U. ; Tang, L. ; Chen, Z. ; Prakash, J. ; Huang, Y. ; Stiel, A.-C. ; Mettenleiter, G. ; Aichler, M. ; Blutke, A. ; Walch, A.K. ; Kleigrewe, K. ; Razansky, D. ; Sattler, M. ; Ntziachristos, V.
Croconaine-based nanoparticles enable efficient optoacoustic imaging of murine brain tumors.Herud-Sikimić, O. ; Stiel, A.-C. ; Kolb, M. ; Shanmugaratnam, S. ; Berendzen, K.W. ; Feldhaus, C. ; Höcker, B. ; Jürgens, G.
A biosensor for the direct visualization of auxin.Cell Engineering: Contact