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Bacterial cloning and expression vectors for the Creator cloning technology

The pETC-K expression vectors are based on pET-24d(+)

Vector Vector map Promoter Antibiotic resistance Tag(s) Expression host Origin of replication Source Description
pDNR-Dual map   Amp   DH5α pUC Clontech donor vector
pDNR-Dual/3C map   Amp   DH5α pUC EMBL-AG donor vector containing 3C protease cleavage site
pDNR-Dual/tev map   Amp   DH5α pUC EMBL-AG donor vector containing TEV protease cleavage site
pETC-10K map T7/lac Kan N-His BL21 (DE3) pBR322 EMBL-AG expression vector
pETC-20K map T7/lac Kan N-TrxA
N-His		 BL21 (DE3) pBR322 EMBL-AG expression vector
pETC-30K map T7/lac Kan N-His
N-GST		 BL21 (DE3) pBR322 EMBL-AG expression vector
pETC-40K map T7/lac Kan N-MBP BL21 (DE3) pBR322 EMBL-AG expression vector
pETC-41K map T7/lac Kan N-His
N-MBP		 BL21 (DE3) pBR322 EMBL-AG expression vector
pETC-50K map T7/lac Kan N-DsbA
N-His		 BL21 (DE3) pBR322 EMBL-AG expression vector
pETC-52K map T7/lac Kan N-leaderless DsbA
N-His		 BL21 (DE3) pBR322 EMBL-AG expression vector
pETC-60K map T7/lac Kan N-NusA
N-His		 BL21 (DE3) pBR322 EMBL-AG expression vector

 

Source

EMBL-AG = EMBL-made vector by Arie Geerlof