Strain database
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Escherichia coli strains and features
Information on antibiotic stock and working concentrations is given below the Strains table.
| Strain | Description | Application | Antibiotic resistance |
|---|---|---|---|
| AD494 (DE3) | thioredoxin reductase (trxB) mutant | trxB expression host; disulfite bond formation in cytoplasm | Kan |
| B834 (DE3) | methionine auxotroph; deficient in lon and ompT proteases | general expression host; labelling of proteins with 35S-methionine and selenomethionine | none |
| B834 (DE3) pLysS | methionine auxotroph; deficient in lon and ompT proteases | high-stringency expression host; labelling of proteins with 35S-methionine and selenomethionine | Cam |
| B834 (DE3) pRARE | methionine auxotroph; deficient in lon and ompT proteases; contains plasmid encoding argU, argW, glyT, ileX, leuW, metT, proL, thrT, thrU & tyrU. | high-stringency expression host; labelling of proteins with 35S-methionine and selenomethionine; allows expression of genes encoding tRNAs for rare argenine codons AGA and AGG, glycine codon GGA, isoleucine codon AUA, leucine codon CUA, methionine codon AUG, proline codon CCC, threonine codons ACA and ACC, and tyrosine codon UAC | Cam |
| BB7333 | deficient in clpX, clpP and lon proteases | general prupose expression host with inhibited protein degradation | Cam (10µg/ml) |
| BL21 | deficient in lon and ompT proteases | general puropose expression host for plasmids which do not use a T7 promoter | none |
| BL21 AI | deficient in lon and ompT proteases | general puropose expression host; expression induced by addition of arabinose | none |
| BL21 SI | deficient in lon and ompT proteases | general puropose expression host; expression induced by addition of NaCl | none |
| BL21 (DE3) | deficient in lon and ompT proteases | general puropose expression host | none |
| BL21 (DE3) cc1 | deficient in lon and ompT proteases; contains plasmids encoding bacterial chaperones | general puropose expression host ; allows expression of genes encoding DnaK, DnaJ and GrpE | Cam (10µg/ml) + Spec |
| BL21 (DE3) cc2 | deficient in lon and ompT proteases; contains plasmids encoding bacterial chaperones | Cam (10µg/ml) + Spec | |
| BL21 (DE3) cc3 | deficient in lon and ompT proteases; contains plasmids encoding bacterial chaperones | general puropose expression host ; allows expression of genes encoding GroEL and GroES | Cam (10µg/ml) + Spec |
| BL21 (DE3) cc4 | deficient in lon and ompT proteases; contains plasmids encoding bacterial chaperones | general puropose expression host ; allows expression of genes encoding DnaK, DnaJ and GrpE, GroEL, GroES and ClpB | Cam (10µg/ml) + Spec |
| BL21 (DE3) cc5 | deficient in lon and ompT proteases; contains plasmids encoding bacterial chaperones | general puropose expression host ; allows expression of genes encoding DnaK, DnaJ and GrpE, GroEL (low), GroES (low) and ClpB | Cam (10µg/ml) + Spec |
| BL21 (DE3) pLysS | deficient in lon and ompT proteases | general puropose high-stringency expression host | Cam |
| BL21 (DE3) pLysE | deficient in lon and ompT proteases | general puropose high-stringency expression host | Cam |
| BL21 (DE3) CodonPlus-RIL | deficient in lon and ompT proteases; contains plasmid encoding argU, ileX and leuW. | exression host; allows expression of genes enpcoding tRNAs for rare argenine codon AGA and AGG, isoleucine codon AUA and leucine codon CUA | Cam |
| BL21 (DE3) CodonPlus-RP | deficient in lon and ompT proteases; contains plasmid encoding argU, and proL. | expression host; allows expression of genes encoding tRNAs for rare argenine codon AGA and AGG and proline codon CCC | Cam |
| BL21 Star (DE3) | RNaseE (rne131) mutant | general puropose expression host with reduced mRNA degradation | none |
| BL21 Star (DE3) pLysS | RNaseE (rne131) mutant | general puropose high-stringency expression host with reduced mRNA degradation | Cam |
| BL21 Star (DE3) pRARE | RNaseE (rne131) mutant; contains plasmid encoding argU, argW, glyT, ileX, leuW, metT, proL, thrT, thrU and tyrU. | general purpose expression host with reduced mRNA degradation; allows expression of genes encoding tRNAs for rare argenine codons AGA and AGG, glycine codon GGA, isoleucine codon AUA, leucine codon CUA, methionine codon AUG, proline codon CCC, threonine codons ACA and ACC, and tyrosine codon UAC | Cam |
| BL21 Star (DE3) pRARE2 | RNaseE (rne131) mutant; contains plasmid encoding argU, argW, argX, glyT, ileX, leuW, metT, proL, thrT, thrU and tyrU. | general purpose expression host with reduced mRNA degradation; allows expression of genes encoding tRNAs for rare argenine codons AGA, AGG, CGA and CGG, glycine codon GGA, isoleucine codon AUA, leucine codon CUA, methionine codon AUG, proline codon CCC, threonine codons ACA and ACC, and tyrosine codon UAC | Cam |
| BLR (DE3) | recA- derivative of BL21 | recA- expression host; recommended for use with tandem repeats | Tet |
| C41 (DE3) | deficient in lon and ompT proteases; ATPase mutant | host for expression of membrane proteins | none |
| C43 (DE3) | deficient in lon and ompT proteases; ATPase mutant | host for expression of membrane proteins | none |
| DB3.1 | none | ||
| DH5α | host for cloning and plasmid propagation | none | |
| DH5α Pro/ pRK1037 | contains plasmid (pRK1037) encoding gene for TVMV protease | expression host; allows expression of TVMV protease | Kan |
| DH10Bac | contains parent bacmid bMON14272 and helper plasmid pMON7124 | host for the generation of recombinant bacmid DNA from pFastBac vectors for use in BEVS technology | Kan + Tet |
| DL41 (DE3) | methionine auxotroph | labeling of proteins with 35S-methionine and selenomethinine | none |
| HMS174 | recA mutant | recA- expression host | Rif |
| HMS174 (DE3) | recA mutant | recA- expression host | Rif |
| HMS174 (DE3) pLysS | recA mutant | recA- high-stringency expression host | Rif + Cam |
| HMS174 (DE3) pLysE | recA mutant | recA- high-stringency expression host | Rif + Cam |
| JM101 | expression host (not with T7 promoter) | none | |
| JM105 | expression host (not with T7 promoter) | none | |
| JM 109 (DE3) | expression host | none | |
| JM110 | dam and dcm mutant | dam and dcm expression host (not with T7 promoter); no methylation of DNA at dam and dcm sites | none |
| LMG194 | ara mutant | expression host used in combination with vectors containing BAD promoter | none |
| M15 [pREP4] | contains helper plasmid pREP4 | expression nused in combination with most pQE vectors (Qiagen) | Kan |
| NovaBlue | host for cloning and plasmid propagation | none | |
| Origami | trxB and gor mutant | expression host (not with T7 promoter); disulfite bond formation in cytoplasm | Kan |
| Origami (DE3) | trxB and gor mutant | expression host; disulfite bond formation in cytoplasm | Kan |
| Origami (DE3) pLysS | trxB and gor mutant | high-stringency expression host; disulfite bond formation in cytoplasm | Kan + Cam |
| Origami (DE3) pLacI | trxB and gor mutant | high-stringency expression host; disulfite bond formation in cytoplasm | Kan + Cam |
| Rosetta | lactose permease (lacY) mutant; protease deficient, contains plasmid encoding argU, argW, glyT, ileX, leuW, metT, proL, thrT, thrU & tyrU. | expression host (not with T7 promoter); allows expression of genes encoding tRNAs for rare argenine codons AGA and AGG, glycine codon GGA, isoleucine codon AUA, leucine codon CUA, methionine codon AUG, proline codon CCC, threonine codons ACA and ACC, and tyrosine codon UAC | Cam |
| Rosetta (DE3) | lactose permease (lacY) mutant; protease deficient, contains plasmid encoding argU, argW, glyT, ileX, leuW, metT, proL, thrT, thrU & tyrU. | expression host; allows expression of genes encoding tRNAs for rare argenine codons AGA and AGG, glycine codon GGA, isoleucine codon AUA, leucine codon CUA, methionine codon AUG, proline codon CCC, threonine codons ACA and ACC, and tyrosine codon UAC | Cam |
| Rosetta (DE3) pLysS | lactose permease (lacY) mutant; protease deficient, contains plasmid encoding argU, argW, glyT, ileX, leuW, metT, proL, thrT, thrU & tyrU. | high-stringency expression host; allows expression of genes encoding tRNAs for rare argenine codons AGA and AGG, glycine codon GGA, isoleucine codon AUA, leucine codon CUA, methionine codon AUG, proline codon CCC, threonine codons ACA and ACC, and tyrosine codon UAC | Cam |
| Rosetta(DE3) pLacI | lactose permease (lacY) mutant; protease deficient, contains plasmid encoding argU, argW, glyT, ileX, leuW, metT, proL, thrT, thrU & tyrU. | high-stringency expression host; allows expression of genes encoding tRNAs for rare argenine codons AGA and AGG, glycine codon GGA, isoleucine codon AUA, leucine codon CUA, methionine codon AUG, proline codon CCC, threonine codons ACA and ACC, and tyrosine codon UAC | Cam |
| Rosetta2 (DE3) | lactose permease (lacY) mutant; deficient in lon and ompT proteases; contains plasmid encoding argU, argW, argX, glyT, ileX, leuW, metT, proL, thrT, thrU and tyrU. | expression host; allows expression of genes encoding tRNAs for rare argenine codons AGA, AGG, CGA and CGG, glycine codon GGA, isoleucine codon AUA, leucine codon CUA, methionine codon AUG, proline codon CCC, threonine codons ACA and ACC, and tyrosine codon UAC | Cam |
| Rosetta2 (DE3) pLysS | lactose permease (lacY) mutant; deficient in lon and ompT proteases; contains plasmid encoding argU, argW, argX, glyT, ileX, leuW, metT, proL, thrT, thrU and tyrU. | high-stringency expression host; allows expression of genes encoding tRNAs for rare argenine codons AGA, AGG, CGA and CGG, glycine codon GGA, isoleucine codon AUA, leucine codon CUA, methionine codon AUG, proline codon CCC, threonine codons ACA and ACC, and tyrosine codon UAC | Cam |
| Rosetta-gami2 (DE3) | lactose permease (lacY) mutant; trxB and gor mutant; deficient in lon and ompT proteases; contains plasmid encoding argU, argW, argX, glyT, ileX, leuW, metT, proL, thrT, thrU and tyrU. | expression host; disulfite bond formation in cytoplasm; allows expression of genes encoding tRNAs for rare argenine codons AGA, AGG, CGA and CGG, glycine codon GGA, isoleucine codon AUA, leucine codon CUA, methionine codon AUG, proline codon CCC, threonine codons ACA and ACC, and tyrosine codon UAC | Cam |
| Rosetta-gami2 (DE3) pLysS | lactose permease (lacY) mutant; trxB and gor mutant; deficient in lon and ompT proteases; contains plasmid encoding argU, argW, argX, glyT, ileX, leuW, metT, proL, thrT, thrU and tyrU. | high-stringency expression host; disulfite bond formation in cytoplasm; allows expression of genes encoding tRNAs for rare argenine codons AGA, AGG, CGA and CGG, glycine codon GGA, isoleucine codon AUA, leucine codon CUA, methionine codon AUG, proline codon CCC, threonine codons ACA and ACC, and tyrosine codon UAC | Cam |
| TB1 | host for pUC plasmids | none | |
| Top10 | ara mutant | host for general purposse cloning and plasmid propagation, expression host used in combination with vectors containing BAD promoter | none |
| Tuner | lactose permease (lacY) mutant | expression host (not with T7 promoter); allows uniform uptake of IPTG | none |
| Tuner (DE3) | lactose permease (lacY) mutant | expression host; allows uniform uptake of IPTG | none |
| Tuner (DE3) pLysS | lactose permease (lacY) mutant | high-stringency expression host; allows uniform uptake of IPTG | Cam |
| Tuner (DE3) pLacI | lactose permease (lacY) mutant | high-stringency expression host; allows uniform uptake of IPTG | Cam |
| XL1-Blue | host for cloning and plasmid propagation | Tet |
Antibiotics stock and working concentrations
| Antibiotic | Abbreviation | Working concentration | Stock solution | Comments | |
|---|---|---|---|---|---|
| Ampicillin | Amp | 100 μg/ml | 100 mg/ml in water | ||
| Carbenicillin | Carb | 100 μg/ml | 100 mg/ml in water | ||
| Chloramphenicol | Cam | 33 μg/ml | 33 mg/ml in ethanol | ||
| Kanamycin | Kan | 30 μg/ml | 30 mg/ml in water | ||
| 100 μg/ml | 100 mg/ml in water | in ZYM 5052 auto-induction medium | |||
| Rifampicin | Rif | 200 μg/ml | 300 mg/ml in water | ||
| Spectinomycin | Spec | 50 μg/ml | 50 mg/ml in water | ||
| Streptomycin | Strep | 50 μg/ml | 50 mg/ml in water | ||
| Tetratcyclin | Tet | 5 μg/ml | 12.5 mg/ml in water |