The Cryoskeleton Lab
Marion Jasnin Lab
Marion’s team explores the diversity of actin structures involved in cell invasion and contractility using cryo-electron tomography. Her group aims to elucidate the structural principles governing actin functions across scales, and to provide an integrated view of the mechanisms of force generation by actin networks in cells.
Marion’s team explores the diversity of actin structures involved in cell invasion and contractility using cryo-electron tomography. Her group aims to elucidate the structural principles governing actin functions across scales, and to provide an integrated view of the mechanisms of force generation by actin networks in cells.
About our Research
Our group uses in situ cryo-electron tomography methodologies, including correlative cryo-focused ion beam milling sample preparation and image processing to reveal actin architectures directly inside the native cellular environment. We explore the diversity of actin structures involved in invasion, including podosomes (Jasnin et al. Nat Commun 2022), invadopodia, and other actin-propelled membrane protrusions, as well as in cell contractility (Burbaum, Schneider et al. Nat Commun 2021). We develop computational tools for in-cell structural analysis of actin organization. For all these exciting projects, we collaborate with international teams of biologists, biophysicists and biochemists to combine structural, molecular, cellular and theoretical approaches.
Immunological synapse – In collaboration with Loïc Dupré (INFINITy/LBI-RUD), Alphée Michelot (Mechanobiology Institute, NUS), Renaud Poincloux (IPBS-Toulouse) and Ralf Jungmann (MPI of Biochemistry)
Macrophage podosomes – In collaboration with Renaud Poincloux (IPBS-Toulouse) and Mira Krendel (SUNY Upstate Medical University)
Tumor cell invadopodia – In collaboration with Philippe Chavrier (Institut Curie)
Clathrin-mediated endocytosis – In collaboration with Florian Wilfling (MPI of Biophysics)
Deep learning-based segmentation tools – In collaboration with Tingying Peng (Helmholtz AI)
Cryo-CLEM labels – In collaboration with Felix Sigmund (TUM & Helmholtz Munich)
Principal Investigators
Publications
Selmi, H. ; Walker, A. ; Balas, L. ; Lucio, M. ; Klotz, M. ; Jeridi, A. ; Burrichter, A.G. ; Conti, D.V. ; Chaffringeon, L. ; Beinsteiner, B. ; Jasnin, M. ; Vanthuyne, N. ; Durand, T. ; Yildirim, A.Ö. ; Stecher, B. ; Debarbieux, L. ; Schmitt-Kopplin, P.
Ornithine lipids from Akkermansia muciniphila are dynamically modulated in colitis and shape macrophage inflammatory responses.Karfusehr, C. ; Eder, M. ; Yang, H.Y. ; Beinsteiner, B. ; Jasnin, M. ; Simmel, F.C.
Self-assembled cell-scale containers made from DNA origami membranes.Merino‐Salomón, A. ; Schneider, J. ; Babl, L. ; Krohn, J.F. ; Sobrinos‐Sanguino, M. ; Schäfer, T. ; Luque-Ortega, J.R. ; Alfonso, C. ; Jimenez, M. ; Jasnin, M. ; Schwille, P. ; Rivas, G.
Crosslinking by ZapD drives the assembly of short FtsZ filaments into toroidal structures in solution.Contact PioneerCampus