Biosynthetic antibiotics | Clean and safe production of bacteriophages to battle antibiotic-resistant bacteria
This work describes the cell-free production, transient protein-modification, and time-resolved proteomics analysis of bacteriophages targeted against clinically relevant bacteria.
Proteins come in many flavors | high-throughput tracking of specific protein isoforms
We have generated a suite of gene reporters, EXSISERS, that self-excise themselves out of the native amino-acid chain of a protein of interest to report on the translation of specific protein isoforms from alternatively spliced mRNA.
The Cut and Restore Protein Trick: Self-Excising Designer Proteins Report Isoform Expression
Our proteome is much bigger than our genome because one gene produces several variants of proteins called protein isoforms, whose disbalance is implicated in many diseases. A new bioengineered reporter system developed at Helmholtz Zentrum München…
What happens in encapsulins stays in encapsulins | bioorthogonal compartments in mammalian cells
To sequester multi-component enzymatic processes inside living mammalian cells, we introduce heterologously expressed encapsulins as a versatile platform to generate self-assembling ompartments which can serve as nano-reaction chambers.
We have synthesized a calcium sensor for photoacoustics, which changes its absorbance and thus photoacoustic spectrum upon selective binding to Ca2+ for future use in organoids or in vivo models.
